[电磁噪声阻断50Hz磁场诱导的缝隙连接细胞间通讯抑制]
[Electromagnetic noise blocks the gap-junctional intercellular communication suppression induced by 50 Hz magnetic field].
作者信息
Zeng Qunli, Chiang Huai, Fu Yiti, Lu Deqiang, Xu Zhengping
机构信息
Bioelectromagnetic Lab., Zhejiang University School of Medicine, Hangzhou 310031, China.
出版信息
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2002 Aug;20(4):243-5.
OBJECTIVE
To explore whether the superposition of an electromagnetic noise can block gap-junctional intercellular communication(GJIC) suppression induced by 50 Hz 0.4 mT extremely low frequency magnetic field(ELF MF).
METHODS
Fibroblast cells of mice(NIH 3T3) were exposed to 0.4 mT ELF MF or(and) electromagnetic noise with the same intensity of MF for 24 h, and the GJIC was determined by using fluorescence recovery after photobleaching(FRAP) analysis, which was performed with a laser-scanning confocal microscope(Leica, Germany).
RESULTS
ELF MF exposure significantly inhibited GJIC with fluorescence recovery rate of 27.67% +/- 5.12% as compared with the control group(45.57% +/- 9.72%) (P < 0.01), while that of ELF MF plus noise group was (52.61% +/- 8.30%), which was significantly different from ELF MF group(P < 0.01), but not from control(P > 0.05).
CONCLUSION
Electromagnetic noise could block the GJIC suppression induced by 50 Hz 0.4 mT MF.
目的
探讨电磁噪声叠加是否能阻断50Hz 0.4mT极低频磁场（ELF MF）诱导的间隙连接细胞间通讯（GJIC）抑制。
方法
将小鼠成纤维细胞（NIH 3T3）暴露于0.4mT ELF MF或（和）相同强度的电磁噪声中24小时，采用激光扫描共聚焦显微镜（德国徕卡）进行光漂白后荧光恢复（FRAP）分析来测定GJIC。
结果
与对照组（45.57%±9.72%）相比，暴露于ELF MF显著抑制GJIC，荧光恢复率为27.67%±5.12%（P<0.01），而ELF MF加噪声组的荧光恢复率为（52.61%±8.30%），与ELF MF组有显著差异（P<0.01），但与对照组无显著差异（P>0.05）。
结论
电磁噪声可阻断50Hz 0.4mT MF诱导的GJIC抑制。
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