Garnis Cathie, Baldwin Corisande, Zhang Lewei, Rosin Miriam P, Lam Wan L
British Columbia Cancer Research Centre, Vancouver, British Columbia, Canada.
Cancer Res. 2003 Dec 15;63(24):8582-5.
Loss of 3p has been associated with oral cancer progression and is common in many cancers. However, regions of alteration on 3p are poorly defined. We have constructed a high-resolution chromosomal array using a tiling set of 535 human bacterial artificial chromosomes that provides near complete coverage of 3p. Array comparative genomic hybridization analysis of 20 microdissected oral squamous cell carcinomas showed multiple and recurrent segments of copy number changes. These include a deletion containing the FHIT gene; novel segments of copy decrease at 3p22, 3p24, and 3p26; and an unexpected approximately 0.7 Mbp segmental increase at 3p21. These data strongly support the value of using chromosomal array comparative genomic hybridization for detailed profiling of oral squamous cell carcinomas.
3p缺失与口腔癌进展相关，且在许多癌症中很常见。然而，3p上的改变区域定义不明确。我们使用一组535个人类细菌人工染色体构建了一个高分辨率染色体阵列，该阵列几乎完全覆盖了3p。对20例显微切割的口腔鳞状细胞癌进行阵列比较基因组杂交分析，结果显示存在多个反复出现的拷贝数变化片段。这些变化包括一个包含FHIT基因的缺失；3p22、3p24和3p26处新的拷贝数减少片段；以及3p21处意外出现的约0.7 Mbp的片段性增加。这些数据有力地支持了使用染色体阵列比较基因组杂交对口腔鳞状细胞癌进行详细分析的价值。
