RNA干扰介导的RITS复合物对异染色质的靶向作用。
RNAi-mediated targeting of heterochromatin by the RITS complex.
作者信息
Verdel André, Jia Songtao, Gerber Scott, Sugiyama Tomoyasu, Gygi Steven, Grewal Shiv I S, Moazed Danesh
机构信息
Department of Cell Biology, Harvard Medical School, Boston, MA02115, USA.
出版信息
Science. 2004 Jan 30;303(5658):672-6. doi: 10.1126/science.1093686. Epub 2004 Jan 2.
Abstract
RNA interference (RNAi) is a widespread silencing mechanism that acts at both the posttranscriptional and transcriptional levels. Here, we describe the purification of an RNAi effector complex termed RITS (RNA-induced initiation of transcriptional gene silencing) that is required for heterochromatin assembly in fission yeast. The RITS complex contains Ago1 (the fission yeast Argonaute homolog), Chp1 (a heterochromatin-associated chromodomain protein), and Tas3 (a novel protein). In addition, the complex contains small RNAs that require the Dicer ribonuclease for their production. These small RNAs are homologous to centromeric repeats and are required for the localization of RITS to heterochromatic domains. The results suggest a mechanism for the role of the RNAi machinery and small RNAs in targeting of heterochromatin complexes and epigenetic gene silencing at specific chromosomal loci.
摘要
RNA干扰（RNAi）是一种广泛存在的沉默机制，作用于转录后和转录水平。在此，我们描述了一种名为RITS（RNA诱导的转录基因沉默起始）的RNAi效应复合物的纯化过程，它是裂殖酵母中异染色质组装所必需的。RITS复合物包含Ago1（裂殖酵母中Argonaute的同源物）、Chp1（一种与异染色质相关的染色质结构域蛋白）和Tas3（一种新蛋白）。此外，该复合物还包含一些小RNA，其产生需要Dicer核糖核酸酶。这些小RNA与着丝粒重复序列同源，是RITS定位于异染色质区域所必需的。这些结果提示了一种机制，说明RNAi机制和小RNA在靶向异染色质复合物以及特定染色体位点的表观遗传基因沉默中的作用。
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