Berczyński Paweł, Kładna Aleksandra, Piechowska Teresa, Kruk Irena, Bozdağ-Dündar Oya, Aboul-Enein Hassan Y, Ceylan-Unlusoy Meltem, Ertan Rahmiye
West Pomeranian University of Technology, Szczecin Institute of Physics, al. Piastów 48, 70-311, Szczecin, Poland.
Luminescence. 2014 Dec;29(8):1107-12. doi: 10.1002/bio.2667. Epub 2014 Apr 14.
A series of flavonyl-2,4-thiazolidinedione, imidazolidinedione and rhodanine derivatives were tested for their antioxidant activity as scavengers of oxygen free radicals. Free radical scavenging activities, including superoxide anion radical O2•, hydroxyl radical (HO(•)) and 2,2'-diphenyl-1-picrylhydrazyl free radical have been evaluated using chemiluminescence, electron paramagnetic resonance and spin trapping with 5,5-dimethyl-1-pyrroline-1-oxide as a spin trap. Potassium superoxide in dimethylsulfoxide and 18-crown-6 ether were used for the production of O2•. Hydroxyl radical was generated using the Fenton reaction. Ten of the eleven examined compounds exhibited decrease in chemiluminescence, but there were large differences in the decrease, ranging from 16% to 89%; also, two of these compounds increased light emission by about 200%. On the contrary, all compounds tested exhibited 30-68% scavenging HO(•) and 25-96% scavenging the DPPH(•) radical respectively. Possible mechanisms are proposed to explain the results.
测试了一系列黄酮基-2,4-噻唑烷二酮、咪唑烷二酮和若丹宁衍生物作为氧自由基清除剂的抗氧化活性。使用化学发光、电子顺磁共振以及以5,5-二甲基-1-吡咯啉-1-氧化物作为自旋捕集剂的自旋捕集技术，评估了包括超氧阴离子自由基O2•、羟基自由基(HO(•))和2,2'-二苯基-1-苦基肼自由基在内的自由基清除活性。使用二甲基亚砜中的超氧化钾和18-冠-6醚来产生O2•。通过芬顿反应产生羟基自由基。所检测的11种化合物中有10种表现出化学发光降低，但降低程度差异很大，从16%到89%不等；此外，其中两种化合物使发光增加了约200%。相反，所有测试化合物分别表现出30 - 68%的HO(•)清除率和25 - 96%的DPPH(•)自由基清除率。提出了可能的机制来解释这些结果。
