Shennan D B
Hannah Research Institute, Scotland, UK.
Placenta. 1992 Sep-Oct;13(5):489-99. doi: 10.1016/0143-4004(92)90054-w.
The efflux of radiolabelled sulphate and chloride from fragments prepared from normal human term placentae has been examined so that a comparison between the transport properties of whole tissue and isolated placental membranes may be made. Sulphate efflux was found via a temperature- and DIDS sensitive mechanism. External chloride and sulphate were able to trans-accelerate the egress of labelled sulphate from tissue fragments via a DIDS-inhibitable route. These results are consistent with sulphate transport being mediated via an anion-exchange process. Chloride efflux from fragments of placenta was via a system which could be trans-stimulated by external Cl-: this moiety of transport was inhibited by DIDS. Several differences between the characteristics of placental tissue anion transport and isolated membrane vesicles were noted and are discussed.
对从正常足月人胎盘制备的组织碎片中放射性标记硫酸盐和氯化物的流出进行了检测，以便比较整个组织和分离的胎盘膜的转运特性。发现硫酸盐流出是通过一种对温度和二异丙基氟磷酸（DIDS）敏感的机制进行的。外部氯化物和硫酸盐能够通过DIDS可抑制的途径加速标记硫酸盐从组织碎片中的流出。这些结果与硫酸盐转运是通过阴离子交换过程介导的一致。胎盘碎片中的氯化物流出是通过一个可被外部Cl-反刺激的系统：这种转运部分被DIDS抑制。注意到并讨论了胎盘组织阴离子转运特性与分离的膜囊泡之间的几个差异。
