泌乳大鼠乳腺组织切片中硫酸盐转运的研究：阴离子交换的证据。

A study of sulphate transport by lactating rat mammary tissue slices: evidence for anion exchange.

Shennan D B

Hannah Research Institute, Ayr, Scotland, UK.

Comp Biochem Physiol A Comp Physiol. 1989;92(1):145-50. doi: 10.1016/0300-9629(89)90758-5.

Abstract

The efflux of radiolabelled sulphate from lactating rat mammary tissue slices has been studied. Sulphate efflux was found to be time- and temperature-dependent. 2. 4,4'-Diisothiocyanostilbene-2,2'-disulphonate (DIDS) inhibited a portion of sulphate release, whereas bumetanide was without effect. 3. The anions chloride, iodide and sulphate trans-stimulated sulphate efflux when added to the incubation medium. The increase in the efflux rate of sulphate found with chloride could be markedly inhibited by DIDS. Thiocyanate, unlike the other anions tested, only had a small effect. 4. The results strongly suggest that there is an anion exchange mechanism in the mammary gland which can mediate the transport of sulphate. This transporter may be important for the metabolism of sulphate by the mammary gland and may also help determine milk anion concentrations.

摘要

对泌乳大鼠乳腺组织切片中放射性标记硫酸盐的外流进行了研究。发现硫酸盐外流具有时间和温度依赖性。2. 4,4'-二异硫氰基芪-2,2'-二磺酸盐（DIDS）抑制了一部分硫酸盐释放，而布美他尼则无作用。3. 当向孵育培养基中添加氯离子、碘离子和硫酸根离子时，它们会反刺激硫酸盐外流。氯离子导致的硫酸盐外流速率增加可被DIDS显著抑制。与其他测试阴离子不同，硫氰酸盐的作用很小。4. 结果强烈表明，乳腺中存在一种阴离子交换机制，可介导硫酸盐的转运。这种转运体可能对乳腺中硫酸盐的代谢很重要，也可能有助于确定乳汁中的阴离子浓度。