Muroyama Yuko, Kondoh Hisato, Takada Shinji
Kondoh Differentiation Signaling Project, Exploratory Research for Advanced Technology (ERATO), Japan Science and Technology Corporation (JST), Kinki Innovation Center, 14 Yoshida-Kawaramachi, Sakyo-ku, Kyoto 606-8305, Japan.
Biochem Biophys Res Commun. 2004 Jan 23;313(4):915-21. doi: 10.1016/j.bbrc.2003.12.023.
Wnt signaling is implicated in the control of cell growth and differentiation during CNS development from studies of mouse and chick models, but its action at the cellular level has been poorly understand. In this study, we examine the in vitro function of Wnt signaling in embryonic neural stem cells, dissociated from neurospheres derived from E11.5 mouse telencephalon. Conditioned media containing active Wnt-3a proteins are added to the neural stem cells and its effect on regeneration of neurospheres and differentiation into neuronal and glial cells was examined. Wnt-3a proteins inhibit regeneration of neurospheres, but promote differentiation into MAP2-positive neuronal cells. Wnt-3a proteins also increase the number of GFAP-positive astrocytes but suppress the number of oligodendroglial lineage cells expressing PDGFR or O4. These results indicate that Wnt-3a signaling can inhibit the maintenance of neural stem cells, but rather promote the differentiation of neural stem cells into several cell lineages.
从小鼠和鸡模型的研究来看，Wnt信号通路参与中枢神经系统发育过程中的细胞生长和分化调控，但其在细胞水平的作用仍知之甚少。在本研究中，我们检测了Wnt信号通路在从E11.5小鼠端脑来源的神经球解离得到的胚胎神经干细胞中的体外功能。将含有活性Wnt-3a蛋白的条件培养基添加到神经干细胞中，并检测其对神经球再生以及向神经元和神经胶质细胞分化的影响。Wnt-3a蛋白抑制神经球再生，但促进向MAP2阳性神经元细胞的分化。Wnt-3a蛋白还增加了GFAP阳性星形胶质细胞的数量，但抑制了表达PDGFR或O4的少突胶质细胞系细胞的数量。这些结果表明，Wnt-3a信号通路可抑制神经干细胞的维持，而是促进神经干细胞向多种细胞谱系的分化。
