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三维培养的神经干细胞在太空飞行中mRNA和miRNA表达的系统分析

Systematic Analysis of mRNA and miRNA Expression of 3D-Cultured Neural Stem Cells (NSCs) in Spaceflight.

作者信息

Cui Yi, Han Jin, Xiao Zhifeng, Qi Yiduo, Zhao Yannan, Chen Bing, Fang Yongxiang, Liu Sumei, Wu Xianming, Dai Jianwu

机构信息

Reproductive and Genetic Center of National Research Institute for Family Planning, Beijing, China.

Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.

出版信息

Front Cell Neurosci. 2018 Jan 11;11:434. doi: 10.3389/fncel.2017.00434. eCollection 2017.

DOI:10.3389/fncel.2017.00434
PMID:29375320
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5768636/
Abstract

Recently, with the development of the space program there are growing concerns about the influence of spaceflight on tissue engineering. The purpose of this study was thus to determine the variations of neural stem cells (NSCs) during spaceflight. RNA-Sequencing (RNA-Seq) based transcriptomic profiling of NSCs identified many differentially expressed mRNAs and miRNAs between space and earth groups. Subsequently, those genes with differential expression were subjected to bioinformatic evaluation using gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes pathway (KEGG) and miRNA-mRNA network analyses. The results showed that NSCs maintain greater stemness ability during spaceflight although the growth rate of NSCs was slowed down. Furthermore, the results indicated that NSCs tended to differentiate into neuron in outer space conditions. Detailed genomic analyses of NSCs during spaceflight will help us to elucidate the molecular mechanisms behind their differentiation and proliferation when they are in outer space.

摘要

最近，随着太空计划的发展，人们越来越关注太空飞行对组织工程的影响。因此，本研究的目的是确定太空飞行期间神经干细胞（NSCs）的变化。基于RNA测序（RNA-Seq）的NSCs转录组分析确定了太空组和地球组之间许多差异表达的mRNA和miRNA。随后，使用基因本体论（GO）、京都基因与基因组百科全书通路（KEGG）和miRNA-mRNA网络分析对那些差异表达的基因进行生物信息学评估。结果表明，尽管NSCs的生长速度减慢，但在太空飞行期间它们保持了更强的干性能力。此外，结果表明NSCs在外太空条件下倾向于分化为神经元。对太空飞行期间NSCs进行详细的基因组分析将有助于我们阐明它们在外太空时分化和增殖背后的分子机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83ef/5768636/c8ca7e630b6a/fncel-11-00434-g007.jpg
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