Golzio M, Gabriel B, Boissier F, Deuwille J, Rols M P, Teissié J
IPBS CNRS (UMR 5089) 205, route de Narbonne, 31077 Toulouse.
J Soc Biol. 2003;197(3):301-10.
Trains of short and intense electric pulses may induce a reversible local permeabilization on the membrane of the treated cells. Hydrophilic species can then almost freely cross the envelope and either enter or escape from the cytoplasm. The purpose of the present study was to investigate the possibility of introducing well defined amounts of Ca2+ ions within the cell. Chinese hamster ovary cells were used as a model system. When the pulsing buffer contained high levels of free Ca2+, the survival of cells was strongly affected. A 1 mM level was well tolerated. When cells were pulsed under moderated field conditions, it was observed that Ca2+ entered cells very rapidly (second time range). But the basic cytoplasmic level was set back spontaneously within a few minutes. The perspectives of this electrical injection are discussed for basic cell biology and high-throughput biotechnology.
短而强的电脉冲序列可在被处理细胞的膜上诱导可逆的局部通透性。亲水性物质随后几乎可以自由穿过包膜，进入或逸出细胞质。本研究的目的是探讨在细胞内引入明确定量的Ca2+离子的可能性。中国仓鼠卵巢细胞被用作模型系统。当脉冲缓冲液中含有高水平的游离Ca2+时，细胞的存活率受到强烈影响。1 mM的水平耐受性良好。当细胞在适度电场条件下接受脉冲处理时，观察到Ca2+非常迅速地（在秒级范围内）进入细胞。但基本的细胞质水平在几分钟内会自发恢复。本文讨论了这种电注射技术在基础细胞生物学和高通量生物技术方面的应用前景。
