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[采用高效液相色谱法测定蚕豆种子成熟过程中的吡喃葡萄糖苷]

[Use of the HPLC method for determination of glucopiranosides during ripening of faba bean seeds].

作者信息

Nestorowicz J, Pierzynowska-Kornik G, Zadernowski R

机构信息

Katedra Technologii Produktów Roślinnych, Wydział Technologii Zywności, Akademia Rolniczo-Techniczna.

出版信息

Rocz Panstw Zakl Hig. 1996;47(2):231-7.

PMID:9064746
Abstract

Uridine (C9H12N2O6) was applied as an internal standard for the determination of glucopiranosides in faba bean seeds by the HPLC method. The relative UV response factors of vaccine or convicine to uridine were determined (1.12 and 0.79, respectively). The changes in the content of vaccine and convicine during ripening of faba bean seeds were followed. It was observed that with the increase in the seed dry matter content from 0.18 kg/kg to 0.78 kg/kg, the dry matter based content of vaccine decreased from 26.225 g/kg to 0.243 g/kg, and that of convicine from 9.051 g/kg to 0.094 g/kg.

摘要

尿苷(C9H12N2O6)被用作内标,采用高效液相色谱法测定蚕豆种子中的吡喃葡萄糖苷。测定了蚕豆嘧啶或伴蚕豆嘧啶对尿苷的相对紫外响应因子(分别为1.12和0.79)。跟踪了蚕豆种子成熟过程中蚕豆嘧啶和伴蚕豆嘧啶含量的变化。观察到,随着种子干物质含量从0.18千克/千克增加到0.78千克/千克,以干物质计的蚕豆嘧啶含量从26.225克/千克降至0.243克/千克,伴蚕豆嘧啶含量从9.051克/千克降至0.094克/千克。

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