一种用于直接测定西非金合欢种子中血清素前体5-羟基色氨酸的高效液相色谱法。

An HPLC method for the direct assay of the serotonin precursor, 5-hydroxytrophan, in seeds of Griffonia simplicifolia.

作者信息

Lemaire Peter A, Adosraku Reimmel K

机构信息

Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA.

出版信息

Phytochem Anal. 2002 Nov-Dec;13(6):333-7. doi: 10.1002/pca.659.

DOI:10.1002/pca.659

PMID:12494751

Abstract

5-Hydroxytryptophan (1) is a naturally occurring amino acid found in significant levels in seeds of Griffonia simplicifolia and used in the treatment of the numerous effects of serotonin deficiency syndrome. An HPLC method has been developed for the direct assay of 1 in seeds of G. simplicifolia which overcomes the problems associated with previous techniques. By optimising the solvent extraction procedures and the HPLC conditions, levels of 1 could be estimated following a single-step seed extraction. The chromatographic conditions, solvent system and the extraction technique developed make this method relatively simple, fast and efficient. Using the described methods, the highest ever levels of 1 (namely, 20.83% on a fresh weight basis) have been determined in seeds of G. simplicifolia obtained in Ghana.

摘要

5-羟色氨酸（1）是一种天然存在的氨基酸，在西非豆（Griffonia simplicifolia）种子中含量较高，可用于治疗血清素缺乏综合征的多种症状。已开发出一种高效液相色谱（HPLC）方法，用于直接测定西非豆种子中的5-羟色氨酸（1），该方法克服了以往技术存在的问题。通过优化溶剂萃取程序和HPLC条件，经过一步种子萃取即可估算出5-羟色氨酸（1）的含量。所开发的色谱条件、溶剂系统和萃取技术使该方法相对简单、快速且高效。使用所述方法，已测定出在加纳获得的西非豆种子中5-羟色氨酸（1）的含量为有史以来最高水平（即鲜重基础上为20.83%）。