Dirnfeld M, Shiloh H, Bider D, Harari E, Koifman M, Lahav-Baratz S, Abramovici H
IVF Unit, Carmel Medical Center, Technion, Rappaport Faculty of Medicine, Haifa, Israel.
Gynecol Endocrinol. 2003 Oct;17(5):397-403. doi: 10.1080/09513590312331290288.
A prospective, randomized study was conducted to evaluate the thickness, of zona pellucida (ZP) after brief or standard exposure of human oocytes to spermatozoa, and to determine the correlation between ZP thickness, fertilization rate and embryo quality. The mean ZP thickness 48 h after insemination was found to be significantly less in fertilized oocytes than in non-fertilized oocytes in all treated groups (13.72 +/- 3.0 microns and 15.08 +/- 2.5 microns, respectively; p < 0.007). Zona pellucida thickness correlated positively with embryo quality. Brief exposure of gametes was found to influence ZP thickness. The ZP was significantly thinner after brief and intracytoplasmic sperm injection (ICSI) exposure of oocytes to spermatozoa than after standard in vitro fertilization (IVF). The mean ZP thickness 24 and 48 h after fertilization was significantly greater in standard IVF (16.43 +/- 2.8 microns and 15.22 +/- 2.7 microns, respectively) than in either the brief exposure or ICSI groups (12.78 +/- 2.4 microns and 13.01 +/- 3.5 microns vs. 13.46 +/- 2.2 microns and 13.16 +/- 2.4 microns; p < 0.0001).
一项前瞻性随机研究旨在评估人类卵母细胞与精子短暂或标准接触后透明带（ZP）的厚度，并确定ZP厚度、受精率和胚胎质量之间的相关性。在所有治疗组中，受精的卵母细胞在授精后48小时的平均ZP厚度显著小于未受精的卵母细胞（分别为13.72±3.0微米和15.08±2.5微米；p<0.007）。透明带厚度与胚胎质量呈正相关。发现配子的短暂接触会影响ZP厚度。卵母细胞与精子短暂接触和卵胞浆内单精子注射（ICSI）后，透明带显著薄于标准体外受精（IVF）后。受精后24小时和48小时，标准IVF组的平均ZP厚度（分别为16.43±2.8微米和15.22±2.7微米）显著大于短暂接触组或ICSI组（分别为12.78±2.4微米和13.01±3.5微米，以及13.46±2.2微米和13.16±2.4微米；p<0.0001）。
