Martínez de Velasco G, Rodero M, Cuéllar C
Department of Parasitology, Faculty of Pharmacy, Complutense University, Pl. Ramón y Cajal s/n., 28040 Madrid, Spain.
Parasite. 2003 Dec;10(4):379-81. doi: 10.1051/parasite/2003104379.
The majority of Kudoa species infect the somatic muscle of fish establishing cysts. As there is no effective method to detect infected fish without destroying them, these parasited fish reach the consumer. We have developed this work to determine whether this parasite contains antigenic compounds capable of provoking an immune response in laboratory animals, in order to consider the possible immunopathological effects in man by the ingestion of Kudoa infected fish. BALB/c mice were injected by the subcutaneous route with the following extracts suspended in aluminium hydroxide: Group 1 (black Kudoa sp. pseudocyst extract), group 2 (white Kudoa sp. pseudocyst extract). Specific IgE levels were measured by ELISA. IgE detected in both groups 1 and 2 showed the possible allergenic nature of some of the components of the parasitic extracts.
多数库道虫属物种感染鱼类的体肌并形成囊肿。由于没有在不破坏鱼类的情况下检测受感染鱼的有效方法，这些被寄生的鱼会流向消费者。我们开展这项研究是为了确定这种寄生虫是否含有能够在实验动物中引发免疫反应的抗原性化合物，以便考虑人类摄入感染库道虫的鱼后可能产生的免疫病理效应。通过皮下途径给BALB/c小鼠注射以下悬浮于氢氧化铝中的提取物：第1组（黑色库道虫属假囊肿提取物），第2组（白色库道虫属假囊肿提取物）。通过酶联免疫吸附测定法测量特异性IgE水平。在第1组和第2组中检测到的IgE表明寄生提取物的某些成分可能具有变应原性。
