Martínez de Velasco G, Rodero M, Zapatero L, Cuéllar C
Departamento de Parasitologia, Facultad de Farmacia, Universidad Complutense, 28040 Madrid, España.
Mem Inst Oswaldo Cruz. 2002 Dec;97(8):1091-5. doi: 10.1590/s0074-02762002000800004. Epub 2003 Jan 20.
The majority of Kudoa species infect the somatic muscle of fish establishing cysts. As there is no effective method to detect infected fish without destroying them these parasited fish reach the consumer. This work was developed to determine whether this parasite contains antigenic compounds capable of provoking an immune response in laboratory animals, in order to consider the possible immunopathological effects in man by the ingestion of Kudoa infected fish. BALB/c mice were injected by the subcutaneous route with the following extracts suspended in aluminium hydroxide: group 1 (black Kudoa sp. pseudocyst extract), group 2 (white Kudoa sp. pseudocyst extract), and group 3 (non-infected hake meat extract). Specific antibody levels were measured by ELISA against homologous and heterologous antigens. The highest responses were obtained from the black Kudoa sp. pseudocyst extract (group 1). The low optic density levels detected in group 3 proved that the results obtained in groups 1 and 2 were a consequence of the parasitic extract injection. The IgG1 was the predominant subclass. IgE detected in groups 1 and 2 showed the possible allergenic nature of some of the components of the parasitic extract. High IgA levels and medium IgG2a and IgG3 levels were obtained in groups 1 and 2. Low IgG2b responses were shown. No cross-reactions between Kudoa sp. pseudocyst extracts and the non-infected hake meat extract were observed.
多数库道虫属物种感染鱼类的体肌并形成囊肿。由于没有在不破坏感染鱼的情况下检测它们的有效方法，这些被寄生的鱼最终流向了消费者。开展这项研究是为了确定这种寄生虫是否含有能够在实验动物中引发免疫反应的抗原性化合物，以便考虑人类摄入感染库道虫的鱼后可能产生的免疫病理效应。通过皮下途径给BALB/c小鼠注射以下悬浮于氢氧化铝中的提取物：第1组（黑色库道虫属假囊肿提取物）、第2组（白色库道虫属假囊肿提取物）和第3组（未感染的无须鳕鱼鱼肉提取物）。通过酶联免疫吸附测定法（ELISA）检测针对同源和异源抗原的特异性抗体水平。黑色库道虫属假囊肿提取物（第1组）产生的反应最强。第3组检测到的低光密度水平证明第1组和第2组所获得的结果是注射寄生虫提取物的结果。IgG1是主要的亚类。在第1组和第2组中检测到IgE，这表明寄生虫提取物的某些成分可能具有致敏性。第1组和第2组中获得了高IgA水平以及中等的IgG2a和IgG3水平。IgG2b反应较低。未观察到库道虫属假囊肿提取物与未感染的无须鳕鱼鱼肉提取物之间的交叉反应。
