Bergman Michael, Salman Hertzel, Djaldetti Meir, Fish Lev, Punsky Igor, Bessler Hanna
Department of Medicine C, Rabin Medical Center, Golda Campus, Petah Tiqva, Israel.
J Nutr Biochem. 2004 Jan;15(1):45-50. doi: 10.1016/j.jnutbio.2003.10.001.
Since oxygen free radicals exert a noxious effect on cell functions, the purpose of the study was to examine the influence of the antioxidant vitamins C and E on the phagocytic capacity, apoptotic death, production of TNFalpha and IL-10 by human peripheral blood cells. In addition, an attempt to find a correlation between the effect of these vitamins on apoptosis and DNA synthesis was carried out. Peripheral white blood cells obtained from 27 healthy volunteers were incubated for 24 hr without and with vitamins C and E at doses extrapolated from clinical practice. Incubation of cells with vit. C caused a significant increase in the number of latex particles internalized by each individual polymorphonuclear cell, but not by monocytes. Both vitamins did not change the number of cells capable for phagocytosis. By the method of propidium iodide staining for detection of apoptosis, incubation of the cells with 0.2 mg/mL vit. C for 24 hrs caused a 39% increase in the percentage of apoptotic cells, as compared to those kept at the same incubation conditions without vitamin. 0.125 mg/mL of vit. E did not affect the percentage of apoptotic cells. On the other hand, applying the caspase-3 method for apoptosis detection, vitamins C and E did not affect the caspase-3 activity. Both vitamins caused an inhibition of 3H-TdR incorporation, which was dose-dependent for vit. C. Concentrations of the vitamins lower than those mentioned above did not alter DNA synthesis. While TNFalpha production was not affected by both vitamins, the spontaneous secretion of IL-10 was dose-dependently reduced by vit. C but remained unaltered following incubation with vit. E. The results, although observed in vitro, might be of importance when those vitamins are administered to healthy subjects.
由于氧自由基对细胞功能产生有害影响，本研究的目的是检测抗氧化维生素C和E对人外周血细胞吞噬能力、凋亡死亡、TNFα和IL - 10产生的影响。此外，还尝试寻找这些维生素对凋亡和DNA合成的影响之间的相关性。从27名健康志愿者获取的外周血白细胞在不添加以及添加从临床实践推断出剂量的维生素C和E的情况下孵育24小时。用维生素C孵育导致每个单个多形核细胞内化的乳胶颗粒数量显著增加，但单核细胞未出现这种情况。两种维生素均未改变具有吞噬能力的细胞数量。通过碘化丙啶染色法检测凋亡，与在相同孵育条件下未添加维生素的细胞相比，用0.2mg/mL维生素C孵育细胞24小时导致凋亡细胞百分比增加39%。0.125mg/mL的维生素E未影响凋亡细胞百分比。另一方面，应用caspase - 3法检测凋亡，维生素C和E均未影响caspase - 3活性。两种维生素均导致3H - TdR掺入受到抑制，维生素C的这种抑制呈剂量依赖性。低于上述浓度的维生素未改变DNA合成。虽然两种维生素均未影响TNFα的产生，但维生素C剂量依赖性地降低了IL - 10的自发分泌，而用维生素E孵育后其分泌保持不变。尽管这些结果是在体外观察到的，但当将这些维生素给予健康受试者时可能具有重要意义。
