Grabarczyk M, Podstawka U, Kopeć-Szlezak J
Zakładu Fizjopatologii Instytutu Hematologii, Warszawie.
Acta Haematol Pol. 1991;22(1):136-44.
The work was undertaken in a trial to protect leukocytes against the toxic effect of fenarimol with vitamin E and C. The experiment was carried out in vitro. These vitamins were chosen since they are the components of the physiological antioxidative mechanism in the cell. The protective effect of the vitamins varied depending on the type of the cell and the studied structure as well as on the dose of the antioxidant used. Improvement of the phagocytic activity of the granulocytes was observed after preincubation with vitamin E and using vitamin C it was possible to achieve control values of the phagocytosis index and per cent of non-phagocytizing granulocytes. Both these vitamins in a satisfactory degree improved the morphological and quantitative pattern of lysosomes in cultured lymphocytes treated with fenarimol. The use of vitamin E in doses of 15 micrograms/ml and vitamin C - 0.5 microgram/ml, made possible achieving of complete normalization of the nucleologram in the lymphocytes. The observed effective protection of the leukocytes against the effects of fenarimol by means of antioxidants suggests that these cells are damaged by the mechanism of lipid peroxidation.
这项工作是在一项用维生素E和C保护白细胞免受氯苯嘧啶醇毒性作用的试验中进行的。实验在体外进行。选择这些维生素是因为它们是细胞生理抗氧化机制的组成部分。维生素的保护作用因细胞类型、研究结构以及所用抗氧化剂的剂量而异。用维生素E预孵育后，观察到粒细胞吞噬活性有所改善，使用维生素C能够使吞噬指数和未吞噬粒细胞百分比达到对照值。这两种维生素都令人满意地改善了用氯苯嘧啶醇处理的培养淋巴细胞中溶酶体的形态和数量模式。使用15微克/毫升的维生素E和0.5微克/毫升的维生素C，能够使淋巴细胞中的核仁图完全正常化。通过抗氧化剂观察到白细胞对氯苯嘧啶醇作用的有效保护，这表明这些细胞是通过脂质过氧化机制受损的。
