Cléry-Barraud Cécile, Gaubert Agnès, Masson Patrick, Vidal Dominique
Département de Toxicologie, Centre de Recherches du Service de Santé des Armées, 38702 La Tronche Cedex, France.
Appl Environ Microbiol. 2004 Jan;70(1):635-7. doi: 10.1128/AEM.70.1.635-637.2004.
Spores of Bacillus anthracis are known to be extremely resistant to heat treatment, irradiation, desiccation, and disinfectants. To determine inactivation kinetics of spores by high pressure, B. anthracis spores of a Sterne strain-derived mutant deficient in the production of the toxin components (strain RP42) were exposed to pressures ranging from 280 to 500 MPa for 10 min to 6 h, combined with temperatures ranging from 20 to 75 degrees C. The combination of heat and pressure resulted in complete destruction of B. anthracis spores, with a D value (exposure time for 90% inactivation of the spore population) of approximately 4 min after pressurization at 500 MPa and 75 degrees C, compared to 160 min at 500 MPa and 20 degrees C and 348 min at atmospheric pressure (0.1 MPa) and 75 degrees C. The use of high pressure for spore inactivation represents a considerable improvement over other available methods of spore inactivation and could be of interest for antigenic spore preparation.
已知炭疽芽孢杆菌的孢子对热处理、辐射、干燥和消毒剂具有极强的抵抗力。为了确定高压对孢子的灭活动力学，将一株源自Sterne菌株的毒素成分生产缺陷型突变体（菌株RP42）的炭疽芽孢杆菌孢子，在280至500兆帕的压力下暴露10分钟至6小时，并结合20至75摄氏度的温度。加热和加压相结合导致炭疽芽孢杆菌孢子完全被破坏，在500兆帕和75摄氏度加压后，D值（使90%的孢子群体失活的暴露时间）约为4分钟，相比之下，在500兆帕和20摄氏度时为160分钟，在大气压（0.1兆帕）和75摄氏度时为348分钟。使用高压进行孢子灭活相对于其他现有的孢子灭活方法有显著改进，并且可能对抗原性孢子制备有意义。
