二氧化碳而非碳酸氢根促进铜锌超氧化物歧化酶加过氧化氢的氧化作用。
CO2, not HCO3-, facilitates oxidations by Cu,Zn superoxide dismutase plus H2O2.
作者信息
Liochev Stefan I, Fridovich Irwin
机构信息
Department of Biochemistry, Duke University Medical Center, Durham, NC 27710, USA.
出版信息
Proc Natl Acad Sci U S A. 2004 Jan 20;101(3):743-4. doi: 10.1073/pnas.0307635100. Epub 2004 Jan 7.
Abstract
The Cu,Zn superoxide dismutase catalyzes HCO(3)(-) -dependent oxidations by H(2)O(2). This activity has been shown to depend on the creation of a bound oxidant at the Cu(II) by interactions with H(2)O(2). The bound oxidant was then thought to oxidize HCO(3)(-) to CO(3)(.-), which diffuses into the bulk solution and there oxidizes diverse substrates. We now find that CO(2) rather than HCO(3)(-) facilitates the peroxidations catalyzed by Cu,Zn superoxide dismutase. This fact was shown by a lag in the rate of peroxidation of NADPH when NaHCO(3)(-) was added last and by a burst in the rate when aqueous CO(2) was added last. Both the lag and the burst were eliminated by carbonic anhydrase.
摘要
铜锌超氧化物歧化酶催化由过氧化氢引发的依赖于碳酸氢根（HCO₃⁻）的氧化反应。这种活性已被证明依赖于通过与过氧化氢相互作用在铜（II）处形成一种结合氧化剂。然后这种结合氧化剂被认为将碳酸氢根氧化为碳酸根（CO₃⁻·），其扩散到本体溶液中并在那里氧化各种底物。我们现在发现，促进铜锌超氧化物歧化酶催化的过氧化反应的是二氧化碳而非碳酸氢根。当最后添加碳酸氢钠（NaHCO₃⁻）时，烟酰胺腺嘌呤二核苷酸磷酸（NADPH）过氧化速率出现滞后，而当最后添加二氧化碳水溶液时速率出现爆发，这一事实证明了这一点。碳酸酐酶消除了滞后和爆发现象。
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