Trenti T, Sternieri E, Ceccarelli D, Gallesi D, Masini A
Clinical Pharmacology and Toxicology Institute, University of Modena, Italy.
Toxicol Lett. 1992 Dec;64-65 Spec No:751-5. doi: 10.1016/0378-4274(92)90257-k.
It has been found that acute ethanol (EtOH) intoxication to rats caused approximately 40% depletion of mitochondrial reduced glutathione (GSH). A GSH reduction of similar extent was also observed after the administration to rats of buthionine sulfoximine (BSO), a specific inhibitor of GSH synthesis. The combined treatment of EtOH plus BSO induced a further mitochondrial GSH decrease up to 70% with respect to control. The presence of lipid hydroperoxides in the mitochondrial membrane was observed whenever an additional oxidative stress was associated to a condition of GSH depletion as in the case of EtOH or EtOH plus BSO. Under these conditions a severe derangement in mitochondrial oxidative functions occurred.
