Liang Danna, Liu Min, Hu Qijing, He Min, Qi Xiaohua, Xu Qiang, Zhou Fucai, Chen Xuehao
School of Horticulture and Plant Protection, Yangzhou University, Yangzhou, Jiangsu 225009, P.R. China.
Sci Rep. 2015 May 11;5:9645. doi: 10.1038/srep09645.
Cucumber, a very important vegetable crop worldwide, is easily damaged by pests. Aphids (Aphis gossypii Glover) are among the most serious pests in cucumber production and often cause severe loss of yield and make fruit quality get worse. Identifying genes that render cucumbers resistant to aphid-induced damage and breeding aphid-resistant cucumber varieties have become the most promising control strategies. In this study, a Illumina Genome Analyzer platform was applied to monitor changes in gene expression in the whole genome of the cucumber cultivar 'EP6392' which is resistant to aphids. Nine DGE libraries were constructed from infected and uninfected leaves. In total, 49 differentially expressed genes related to cucumber aphid resistance were screened during the treatment period. These genes are mainly associated with signal transduction, plant-pathogen interactions, flavonoid biosynthesis, amino acid metabolism and sugar metabolism pathways. Eight of the 49 genes may be associated with aphid resistance. Finally, expression of 9 randomly selected genes was evaluated by qRT-PCR to verify the results for the tag-mapped genes. With the exception of 1-aminocyclopropane-1-carboxylate oxidase homolog 6, the expression of the chosen genes was in agreement with the results of the tag-sequencing analysis patterns.
黄瓜是全球一种非常重要的蔬菜作物，很容易受到害虫侵害。蚜虫（棉蚜）是黄瓜生产中最严重的害虫之一，常常导致严重的产量损失并使果实品质变差。鉴定使黄瓜对蚜虫诱导的损害具有抗性的基因以及培育抗蚜虫黄瓜品种已成为最具前景的防治策略。在本研究中，应用Illumina基因组分析仪平台监测抗蚜虫黄瓜品种‘EP6392’全基因组中的基因表达变化。从受感染和未受感染的叶片构建了9个数字基因表达谱文库。在处理期间总共筛选出49个与黄瓜抗蚜虫性相关的差异表达基因。这些基因主要与信号转导、植物-病原体相互作用、类黄酮生物合成、氨基酸代谢和糖代谢途径相关。49个基因中的8个可能与抗蚜虫性有关。最后，通过实时定量聚合酶链反应评估随机选择的9个基因的表达，以验证标签定位基因的结果。除了1-氨基环丙烷-1-羧酸氧化酶同源物6外，所选基因的表达与标签测序分析模式的结果一致。
