Porowski Tadeusz, Gałasiński Władysław
Clinic of Children Diseases Medical Academy, 15-274 Bialystok, 17 Waszyngtona Str., Poland.
Acta Pol Pharm. 2003 Jul-Aug;60(4):239-45.
An enzymatic semi-micromethod for oxalate determination in human plasma was elaborated. The principle of the method depends on the oxalate isolation from deproteinized plasma, following determination by the calorimetric oxalate oxidase-peroxidase-indamine system. This method protects against internal oxalate losses and excludes an interference of contaminations. Results, obtained by this method, were reliable and ideally suited for use as real normal values (less than or equal to 6 microM) of oxalate content in the plasma of healthy individuals. The elaborated method, which can assay plasma oxalate accurately in normal individuals as well as in hyperoxalemic conditions is superior to those previously used. The procedure of semi-micromethod does not require expensive equipments and apparatus: it is simple and easy to perform in every laboratory and takes little time.
阐述了一种用于测定人血浆中草酸盐的酶促半微量法。该方法的原理是先从脱蛋白血浆中分离出草酸盐，然后通过比色法的草酸盐氧化酶-过氧化物酶-吲哚胺系统进行测定。此方法可防止草酸盐的内部损失，并排除污染物的干扰。用该方法获得的结果可靠，非常适合用作健康个体血浆中草酸盐含量的实际正常值（小于或等于6微摩尔）。所阐述的方法能够在正常个体以及高草酸血症情况下准确测定血浆草酸盐，优于先前使用的方法。半微量法的操作过程不需要昂贵的设备和仪器：它简单易行，在每个实验室都能轻松完成，且耗时短。
