血清草酸盐的酶法测定
Enzymic determination of serum oxalate.
作者信息
Sugiura M, Yamamura H, Hirano K, Ito Y, Sasaki M, Morikawa M, Inoue M, Tsuboi M
出版信息
Clin Chim Acta. 1980 Aug 19;105(3):393-9. doi: 10.1016/0009-8981(80)90121-7.
A new colorimetric, enzymic method for determination of serum oxalate is described using oxalate oxidase from barley seedlings. This method, based on a specific reaction of oxalate with oxidase is rapid, simple and precise. The percentage recoveries of oxalate added to serum ranged from 91.5 to 103. The oxalate content in the serum obtained from 50 healthy non-fasting donors varied from 0.06 mg/dl to 0.41 mg/dl. The proposed method showed a good correlation with a chemical method.
描述了一种使用大麦幼苗草酸氧化酶测定血清草酸的新的比色酶法。该方法基于草酸与氧化酶的特异性反应，快速、简单且精确。添加到血清中的草酸回收率在91.5%至103%之间。从50名健康非空腹献血者获得的血清中草酸含量在0.06毫克/分升至0.41毫克/分升之间。所提出的方法与化学方法显示出良好的相关性。
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