Barth Stephan W, Riediger Thomas, Lutz Thomas A, Rechkemmer Gerhard
Federal Research Centre for Nutrition, Institute of Nutritional Physiology, Haid und Neu Str. 9, 76131 Karlsruhe, Germany.
Brain Res. 2004 Jan 30;997(1):97-102. doi: 10.1016/j.brainres.2003.10.040.
The pancreatic hormone amylin (AMY) and the AMY-receptor-agonist salmon-calcitonin (sCT) reduce short-term food-intake after binding to the area postrema (AP), a circumventricular organ (CVO) lacking blood-brain-barrier characteristics. AMY has also been proposed to induce drinking via another CVO, the subfornical organ (SFO). In cellular systems, AMY-binding is generated by interaction of calcitonin-receptor a/b (CT((a))/CT((b))) with receptor-activity modifying proteins (RAMPs). By using in situ hybridization, the codistribution of CT((a))/CT((b)) with RAMP1-3 and c-fos was mapped in CVOs of rats. AMY and sCT induced c-fos within the SFO which contained CT((a)) and/or CT((b)) and RAMP1/2 mRNA. AMY and sCT also activated AP neurons, which express the CT((a)), but not the CT((b)), receptor and RAMP2/3 mRNA. These data emphasize the important role of these structures as primary targets for circulating AMY.
胰腺激素胰岛淀粉样多肽（AMY）和AMY受体激动剂鲑鱼降钙素（sCT）与最后区（AP）结合后可减少短期食物摄入量，最后区是一个缺乏血脑屏障特征的室周器官（CVO）。也有研究提出，AMY可通过另一个室周器官——穹窿下器官（SFO）诱导饮水。在细胞系统中，AMY结合是由降钙素受体a/b（CT（a）/CT（b））与受体活性修饰蛋白（RAMPs）相互作用产生的。通过原位杂交，在大鼠的室周器官中绘制了CT（a）/CT（b）与RAMP1 - 3和c - fos的共分布情况。AMY和sCT在含有CT（a）和/或CT（b）以及RAMP1/2 mRNA的SFO内诱导c - fos表达。AMY和sCT还激活了表达CT（a）但不表达CT（b）受体以及RAMP2/3 mRNA的AP神经元。这些数据强调了这些结构作为循环AMY主要靶点的重要作用。
