用于检测利什曼原虫特异性犬抗体的试纸条检测法的开发。
Development of a dipstick assay for detection of Leishmania-specific canine antibodies.
作者信息
Schallig Henk D F H, Cardoso Luís, Hommers Marieke, Kroon Nel, Belling Guus, Rodrigues Manuela, Semião-Santos Saul J, Vetter Hans
机构信息
KIT (Koninklijk Instituut voor de Tropen/Royal Tropical Institute), KIT Biomedical Research, Amsterdam, The Netherlands.
出版信息
J Clin Microbiol. 2004 Jan;42(1):193-7. doi: 10.1128/JCM.42.1.193-197.2004.
Abstract
A dipstick assay, based on Leishmania infantum antigen, for the rapid detection of Leishmania-specific antibodies in canine serum samples was developed and evaluated. After determination of optimal dipstick test conditions, test performance was compared with two existing serological tests, i.e., the direct agglutination test (DAT) and the fast agglutination screening test (FAST). In the present study the dipstick test had a sensitivity of 99.2% and a specificity of 87.9%. The DAT had a sensitivity of 97.7% and a specificity of 95.2%, whereas the FAST had also a sensitivity of 97.7% and a specificity of 93.0%. High degrees of agreement were observed between the dipstick test and DAT (93.7%; kappa value, 0.86), between the dipstick test and FAST (91.8%; kappa value, 0.82), and between the DAT and FAST (95.2%; kappa value, 0.90). The high sensitivity and ease of performance make the dipstick test very suitable for surveillance surveys.
摘要
开发并评估了一种基于婴儿利什曼原虫抗原的试纸条检测法，用于快速检测犬血清样本中的利什曼原虫特异性抗体。确定最佳试纸条检测条件后，将检测性能与两种现有的血清学检测方法进行比较，即直接凝集试验（DAT）和快速凝集筛查试验（FAST）。在本研究中，试纸条检测法的灵敏度为99.2%，特异性为87.9%。DAT的灵敏度为97.7%，特异性为95.2%，而FAST的灵敏度也为97.7%，特异性为93.0%。试纸条检测法与DAT之间（93.7%；kappa值为0.86）、试纸条检测法与FAST之间（91.8%；kappa值为0.82）以及DAT与FAST之间（95.2%；kappa值为0.90）均观察到高度一致性。高灵敏度和易于操作使得试纸条检测法非常适合用于监测调查。
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