将结核分枝杆菌基因分型方法——寡核苷酸分型法，从基于反向线杂交的膜法检测转换至Luminex多分析物谱系统。
Transfer of a Mycobacterium tuberculosis genotyping method, Spoligotyping, from a reverse line-blot hybridization, membrane-based assay to the Luminex multianalyte profiling system.
作者信息
Cowan Lauren S, Diem Lois, Brake Mary Catherine, Crawford Jack T
机构信息
Division of AIDS, STD, and TB Laboratory Research, National Center for HIV, STD and TB Prevention, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
出版信息
J Clin Microbiol. 2004 Jan;42(1):474-7. doi: 10.1128/JCM.42.1.474-477.2004.
Abstract
Spoligotyping using Luminex technology was shown to be a highly reproducible method suitable for high-throughput analysis. Spoligotyping of 48 isolates using the traditional membrane-based assay and the Luminex assay yielded concordant results for all isolates. The Luminex platform provides greater flexibility and cost effectiveness than the membrane-based assay.
摘要
使用Luminex技术的寡核苷酸分型被证明是一种高度可重复的方法，适用于高通量分析。使用传统的基于膜的检测方法和Luminex检测方法对48株分离株进行寡核苷酸分型，所有分离株的结果均一致。与基于膜的检测方法相比，Luminex平台具有更大的灵活性和成本效益。
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Transfer of a Mycobacterium tuberculosis genotyping method, Spoligotyping, from a reverse line-blot hybridization, membrane-based assay to the Luminex multianalyte profiling system.将结核分枝杆菌基因分型方法——寡核苷酸分型法，从基于反向线杂交的膜法检测转换至Luminex多分析物谱系统。
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本文引用的文献
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