Janakiraman Anuradha, Goldberg Marcia B
Division of Infectious Diseases, Department of Medicine, Massachusetts General Hospital/Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA.
Proc Natl Acad Sci U S A. 2004 Jan 20;101(3):835-40. doi: 10.1073/pnas.0305747101. Epub 2004 Jan 8.
We present evidence that, in Escherichia coli, polar positional information is present at midcell independent of known cell division factors. In filamented cells, IcsA, which is normally polar, localizes at or near potential cell division sites. Because the cell pole is derived from the septum, the sites to which IcsA localizes in filaments correspond to future poles. IcsA localization to these sites is independent of FtsZ, MinCDE, septation, and nucleoid occlusion, indicating that positional information for the future pole is independent of cell division and chromosome positioning. Upon IcsA localization to these sites, septation is inhibited, suggesting that IcsA recognition of this polar positional information may influence cell division.
我们提供的证据表明，在大肠杆菌中，极性位置信息存在于细胞中部，独立于已知的细胞分裂因子。在丝状细胞中，通常位于极性的IcsA定位于潜在的细胞分裂位点或其附近。由于细胞极源自隔膜，IcsA在丝状细胞中定位的位点对应于未来的极。IcsA定位于这些位点独立于FtsZ、MinCDE、隔膜形成和类核阻隔，这表明未来极的位置信息独立于细胞分裂和染色体定位。当IcsA定位于这些位点时，隔膜形成受到抑制，这表明IcsA对这种极性位置信息的识别可能影响细胞分裂。
