果蝇核糖核酸酶Z在体内加工线粒体和细胞核前体tRNA的3'末端。
Drosophila RNase Z processes mitochondrial and nuclear pre-tRNA 3' ends in vivo.
作者信息
Dubrovsky Edward B, Dubrovskaya Veronica A, Levinger Louis, Schiffer Steffen, Marchfelder Anita
机构信息
Department of Biology, Dartmouth College, Hanover, NH 03755, USA.
出版信息
Nucleic Acids Res. 2004 Jan 9;32(1):255-62. doi: 10.1093/nar/gkh182. Print 2004.
Abstract
Although correct tRNA 3' ends are crucial for protein biosynthesis, generation of mature tRNA 3' ends in eukaryotes is poorly understood and has so far only been investigated in vitro. We report here for the first time that eukaryotic tRNA 3' end maturation is catalysed by the endonuclease RNase Z in vivo. Silencing of the JhI-1 gene (RNase Z homolog) in vivo with RNAi in Drosophila S2 cultured cells causes accumulation of nuclear and mitochondrial pre-tRNAs, suggesting that JhI-1 encodes both forms of the tRNA 3' endonuclease RNase Z, and establishing its biological role in endonucleolytic tRNA 3' end processing. In addition our data show that in vivo 5' processing of nuclear and mitochondrial pre-tRNAs occurs before 3' processing.
摘要
尽管正确的tRNA 3'末端对蛋白质生物合成至关重要，但真核生物中成熟tRNA 3'末端的生成却知之甚少，迄今为止仅在体外进行过研究。我们在此首次报道，真核生物tRNA 3'末端成熟在体内由核酸内切酶RNase Z催化。在果蝇S2培养细胞中通过RNA干扰在体内沉默JhI-1基因（RNase Z同源物）会导致细胞核和线粒体前体tRNA的积累，这表明JhI-1编码tRNA 3'核酸内切酶RNase Z的两种形式，并确定了其在核酸内切tRNA 3'末端加工中的生物学作用。此外，我们的数据表明，细胞核和线粒体前体tRNA的体内5'加工发生在3'加工之前。
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