Borda J T, Pauley D R, MacKey J J, Alvarez X, Simon M A, Klumpp S A
Division of Comparative Pathology, Tulane National Primate Research Center, Tulane University, 18703 Three River Road, Covington, LA 70433, USA.
Vet Pathol. 2004 Jan;41(1):44-9. doi: 10.1354/vp.41-1-44.
A 4-year-old female pigtailed macaque (Macaca nemestrina), experimentally coinfected with simian immunodeficiency virus (SIVmac251) and Mycobacterium bovis(bacillus Calmette-Guerin), was euthanatized 1 year after infection because of weight loss and labored breathing. On gross examination, both kidneys were found to be markedly enlarged (right: 54.7 g and left: 51.7 g; normal < 20 g). Renal lesions were evaluated by histopathologic, immunohistochemical, and ultrastructural methods. Light microscopy revealed that the glomeruli were diffusely hypercellular with expansion of the mesangial matrix, and crescent formation affected approximately 60% of the glomeruli. By immunohistochemical evaluation, it was found that the crescents were composed principally of macrophages, as seen by CD68 (KP1), MRP8, MAC387, and HAM56 expression. Electron microscopic examination of the glomeruli revealed extensive intramembranous, subendothelial, and mesangial electron-dense deposits and multifocal fusion of the visceral epithelial foot processes. Immunofluorescence, used to determine the composition of the electron-dense deposits, revealed diffuse granular mesangial and capillary staining for immunoglobulin A (IgA). The renal changes described in this case report are most consistent with the findings of crescentic gloerulonephritis with IgA immune complex deposition in the glomerular basement membrane and mesangium as described in humans with IgA nephropathy.
一只4岁雌性豚尾猕猴（食蟹猴），实验性感染了猿猴免疫缺陷病毒（SIVmac251）和牛分枝杆菌（卡介苗），在感染1年后因体重减轻和呼吸困难而实施安乐死。大体检查发现，双肾均明显肿大（右肾：54.7克，左肾：51.7克；正常<20克）。通过组织病理学、免疫组织化学和超微结构方法对肾脏病变进行评估。光镜检查显示，肾小球弥漫性细胞增多，系膜基质扩张，约60%的肾小球有新月体形成。通过免疫组织化学评估发现，新月体主要由巨噬细胞组成，如CD68（KP1）、MRP8、MAC387和HAM56表达所示。肾小球的电子显微镜检查显示广泛的膜内、内皮下和系膜电子致密沉积物以及脏层上皮足突的多灶性融合。用于确定电子致密沉积物组成的免疫荧光显示，免疫球蛋白A（IgA）呈弥漫性颗粒状系膜和毛细血管染色。本病例报告中描述的肾脏变化与人类IgA肾病中肾小球基底膜和系膜中IgA免疫复合物沉积的新月体性肾小球肾炎的表现最为一致。
