Yamada M, Nakamura K, Yoshii M, Kaku Y
Laboratory of Chronic Disease Pathology, National Institute of Animal Health, Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
Vet Pathol. 2004 Jan;41(1):62-7. doi: 10.1354/vp.41-1-62.
Nonsuppurative encephalitis was experimentally induced in 3-week-old piglets by a single intravenous inoculation of either of two strains (IB 2001 or AS-6) of Japanese encephalitis flavivirus (JEV) isolated from field pigs. The lesions, which consisted of neuronal necrosis, neuronophagia, glial nodules, and perivas-cular cuffing, were distributed in the cerebrum, midbrain, pons, medulla oblongata, and cerebellum, particularly in the gray matter of the frontal and temporal lobes and thalamus. The gray matter of the spinal cord of piglets that were given the AS-6 strain also was affected. JEV antigen was immunohistochemically detected in the cytoplasm of the nerve cells in the cortex of the frontal and temporal lobes and in the gray matter of the thalamus and midbrain. Two JEV strains isolated from field pigs exhibited neurovirulence, inducing nonsup-purative encephalitis in piglets.
通过单次静脉注射从野外猪分离出的两种日本脑炎黄病毒（JEV）毒株（IB 2001或AS-6）之一，在3周龄仔猪中实验性诱导非化脓性脑炎。病变包括神经元坏死、噬神经细胞现象、胶质结节和血管周围套袖状浸润，分布于大脑、中脑、脑桥、延髓和小脑，尤其在额叶和颞叶以及丘脑的灰质中。接种AS-6毒株的仔猪脊髓灰质也受到影响。在额叶和颞叶皮质以及丘脑和中脑灰质的神经细胞胞质中通过免疫组织化学检测到JEV抗原。从野外猪分离出的两种JEV毒株均表现出神经毒力，可在仔猪中诱导非化脓性脑炎。
