Baltaci Abdülkerim Kasim, Bediz Cem Seref, Mogulkoc Rasim, Kurtoglu Erdal, Pekel Aysel
Department of Physiology, Meram Medical School of Selcuk University, 42080 Konya, Turkey.
Biol Trace Elem Res. 2003 Winter;96(1-3):237-45. doi: 10.1385/BTER:96:1-3:237.
The effects of zinc (Zn) and/or melatonin supplementation on cellular immunity were investigated in rats infested with Toxoplasma gondii. Fifty Sprague-Dawley male rats were used for this study. All animals were fed a normal diet, ad libitum, containing 97 mg Zn/kg. They were divided into five experimental groups, as follows. Group I (n = 10) received intraperitoneal injections of zinc sulfate at a dose of 3 mg/kg/d for 3 wk. Group II (n = 10) received intraperitoneal injections of melatonin at a dose of 3 mg/kg/d for 3 wk. Group III (n = 10) received intraperitoneal injections of zinc sulfate (3 mg/kg/d) and melatonin (3 mg/kg/d) for 3 wk. Group IV (n = 10) was infested controls. Group V (n = 10) was healthy controls. There were no differences in the percentage of CD3+ lymphocytes among all groups. For groups I-III, the CD4+ and CD8+ ratios were higher than those of the groups IV and V controls (p<0.01). Similarly, the total lymphocyte ratios in groups I-III were higher than those of infested and healthy controls (p<0.01). The total lymphocyte ratios in group III were significantly higher than those of groups I and II (p<0.01). The plasma Zn levels in the supplemented groups were significantly higher than those of control groups IV and V (p<0.01). These results suggest that melatonin and/or Zn supplementation may activate cellular immunity by stimulating CD4+ and CD8+ production in infected rats with T. gondii.
在感染刚地弓形虫的大鼠中研究了补充锌（Zn）和/或褪黑素对细胞免疫的影响。本研究使用了50只雄性Sprague-Dawley大鼠。所有动物随意进食正常饮食，饮食中含97 mg Zn/kg。它们被分为五个实验组，如下。第一组（n = 10）腹腔注射硫酸锌，剂量为3 mg/kg/d，持续3周。第二组（n = 10）腹腔注射褪黑素，剂量为3 mg/kg/d，持续3周。第三组（n = 10）腹腔注射硫酸锌（3 mg/kg/d）和褪黑素（3 mg/kg/d），持续3周。第四组（n = 10）为感染对照组。第五组（n = 10）为健康对照组。所有组之间CD3 +淋巴细胞百分比无差异。对于第一至三组，CD4 +和CD8 +比率高于第四组和第五组对照组（p<0.01）。同样，第一至三组的总淋巴细胞比率高于感染和健康对照组（p<0.01）。第三组的总淋巴细胞比率显著高于第一组和第二组（p<0.01）。补充组的血浆锌水平显著高于对照组第四组和第五组（p<0.01）。这些结果表明，补充褪黑素和/或锌可能通过刺激感染刚地弓形虫的大鼠产生CD4 +和CD8 +来激活细胞免疫。
