Kondo A, Ueda M
Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe 657-8501, Japan.
Appl Microbiol Biotechnol. 2004 Mar;64(1):28-40. doi: 10.1007/s00253-003-1492-3. Epub 2004 Jan 10.
In a cell-surface engineering system established using the yeast Saccharomyces cerevisiae, novel, so-called arming yeasts are constructed that are armed with biocatalysts in the form of enzymes, functional proteins, antibodies, and combinatorial protein libraries. Among the many advantages of the system, in which proteins are genetically displayed on the cell surface, are easy reproduction of the displayed biocatalysts and easy separation of product from catalyst. As proteins and peptides of various kinds can be displayed on the yeast cell surface, the system is expected to allow the preparation of tailor-made functional proteins. With its ability to express many of the functional proteins necessary for post-translational modification and in a range of different sizes, the yeast-based molecular display system appears uniquely useful among the various display systems so far developed. Capable of conferring novel additional abilities upon living cells, cell-surface engineering heralds a new era of combinatorial bioengineering in the field of biotechnology. This mini-review describes molecular display using yeast and its various applications.
在利用酿酒酵母建立的细胞表面工程系统中，构建了新型的所谓“武装酵母”，这些酵母配备了酶、功能蛋白、抗体和组合蛋白文库等形式的生物催化剂。在该系统的诸多优势中，蛋白质在细胞表面进行基因展示，具有展示的生物催化剂易于复制以及产物易于与催化剂分离的特点。由于各种蛋白质和肽都可以展示在酵母细胞表面，该系统有望用于制备定制的功能蛋白。基于酵母的分子展示系统能够表达许多翻译后修饰所需的功能蛋白，且大小范围多样，在目前已开发的各种展示系统中显得尤为有用。细胞表面工程能够赋予活细胞新的额外能力，开创了生物技术领域组合生物工程的新纪元。这篇小型综述描述了利用酵母的分子展示及其各种应用。
