Li Suhua, Jiang Xuehua, Yang Qiang, Jin Zhaohui
West China School of Pharmacy, Sichuan University, Chengdu 610041.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2003 Dec;20(4):692-4.
An RP-HPLC was established to determine scutellarin in rabbit plasma and to study pharmacokinetics of breviscapine in rabbits. The analytical column was DiamonsilTM C18(4.6 mm x 250 mm, 5 microns), the mobile phase consisted of methanol, 0.02 mol/L phosphate buffer(adjusted by phosphoric acid to pH 3.0) and acetonitrile (65:35:10), the flow rate was 1.0 ml.min-1, the column temperature was 25 degrees C and the detection wavelength was 334 nm. The results of the pharmacokinetics study showed that the concentration-time curve of scutellarin was conformed to two-compartment model, the chief pharmacokinetic parameters were as follows: t1/2 alpha 1.29 +/- 0.53 min, t1/2 beta 10.40 +/- 1.97 min, Vc148.1 +/- 118.6 ml, and CL 57.5 +/- 31.7 ml.min-1.
建立了反相高效液相色谱法（RP-HPLC）测定兔血浆中灯盏花素，并研究灯盏花素在兔体内的药代动力学。分析柱为迪马硅烷C18（4.6mm×250mm，5μm），流动相由甲醇、0.02mol/L磷酸盐缓冲液（用磷酸调至pH3.0）和乙腈（65:35:10）组成，流速为1.0ml·min-1，柱温为25℃，检测波长为334nm。药代动力学研究结果表明，灯盏花素的浓度-时间曲线符合二室模型，主要药代动力学参数如下：t1/2α为1.29±0.53分钟，t1/2β为10.40±1.97分钟，Vc为148.1±118.6ml，CL为57.5±31.7ml·min-1。
