[灯盏花素在兔体内的药代动力学]

[Pharmacokinetics of scutellarin in rabbits].

作者信息

Li Suhua, Jiang Xuehua, Yang Qiang, Jin Zhaohui

机构信息

West China School of Pharmacy, Sichuan University, Chengdu 610041.

出版信息

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2003 Dec;20(4):692-4.

PMID:14716879

Abstract

An RP-HPLC was established to determine scutellarin in rabbit plasma and to study pharmacokinetics of breviscapine in rabbits. The analytical column was DiamonsilTM C18(4.6 mm x 250 mm, 5 microns), the mobile phase consisted of methanol, 0.02 mol/L phosphate buffer(adjusted by phosphoric acid to pH 3.0) and acetonitrile (65:35:10), the flow rate was 1.0 ml.min-1, the column temperature was 25 degrees C and the detection wavelength was 334 nm. The results of the pharmacokinetics study showed that the concentration-time curve of scutellarin was conformed to two-compartment model, the chief pharmacokinetic parameters were as follows: t1/2 alpha 1.29 +/- 0.53 min, t1/2 beta 10.40 +/- 1.97 min, Vc148.1 +/- 118.6 ml, and CL 57.5 +/- 31.7 ml.min-1.

摘要

建立了反相高效液相色谱法（RP-HPLC）测定兔血浆中灯盏花素，并研究灯盏花素在兔体内的药代动力学。分析柱为迪马硅烷C18（4.6mm×250mm，5μm），流动相由甲醇、0.02mol/L磷酸盐缓冲液（用磷酸调至pH3.0）和乙腈（65:35:10）组成，流速为1.0ml·min-1，柱温为25℃，检测波长为334nm。药代动力学研究结果表明，灯盏花素的浓度-时间曲线符合二室模型，主要药代动力学参数如下：t1/2α为1.29±0.53分钟，t1/2β为10.40±1.97分钟，Vc为148.1±118.6ml，CL为57.5±31.7ml·min-1。