Che Qing-Ming, Chen Ying, Pan Li-Yi, He Hong
School of Pharmaceutical Sciences, Beijing University, Beijing 100083, China.
Zhongguo Zhong Yao Za Zhi. 2006 Oct;31(20):1710-2.
A HPLC-ECD method was established to determine scutellarin in rat bile.
The analytical column was Prontosil C18 (4.6 mm x 250 mm, 5 microm), and the mobile phase was consisted of methanol, 50 mmol x L(-1) phosphate buffer (adjusted by phosphoric acid to pH 2. 6), and tetrahydrofuran (40: 60: 10) , the flow rate was 1.0 mL x min(-1); the potential electrode voltage was 100 mv.
Concentration profile of scutellarin in rat bile was shown in this paper after oral administration of scutellarein.
Only scutellarin was detected in rat bile, while both of scutellarin and scutellarein were detected in rat plasma.
建立一种高效液相色谱-电化学检测法测定大鼠胆汁中的野黄芩苷。
分析柱为普洛帝C18（4.6mm×250mm，5μm），流动相由甲醇、50mmol·L⁻¹磷酸盐缓冲液（用磷酸调至pH 2.6）和四氢呋喃（40:60:10）组成，流速为1.0mL·min⁻¹；电极电位为100mV。
本文给出了大鼠口服黄芩素后胆汁中野黄芩苷的浓度曲线。
在大鼠胆汁中仅检测到野黄芩苷，而在大鼠血浆中同时检测到野黄芩苷和黄芩素。
