Funovics Martin A, Alencar Herlen, Su Henry S, Khazaie Khashayarsha, Weissleder Ralph, Mahmood Umar
Massachusetts General Hospital, Boston 02125, USA.
Mol Imaging. 2003 Oct;2(4):350-7. doi: 10.1162/15353500200303166.
We describe the design and construction of a miniaturized multichannel near infrared (NIR) endoscopic imaging system developed for high-resolution imaging of mice. The device allows for simultaneous real-time video images in white light and two independent NIR channels. Testing demonstrated independent acquisition of nanomolar concentrations of fluorochromes Cy5.5 and Cy7. Cross-talk between the NIR channels, partially a result of broad tails in the spectra of commonly used organic fluorochromes, was assessed, modeled for the linear range of the concentration/signal intensity function, and compensated. The calculated compensation was 5.5% and 22% of the total signal intensity in the two channels NIR700 and NIR780, respectively, at equal concentrations of the two fluorochromes. Using a mouse model of colonic adenomatosis, we show that both perfusion and protease activity can be detected simultaneously, independently, and repeatedly in live mice. The developed device should be useful for in vivo imaging of diverse molecular targets.
我们描述了一种为小鼠高分辨率成像而开发的小型化多通道近红外（NIR）内镜成像系统的设计与构建。该设备能够同时提供白光下的实时视频图像以及两个独立的近红外通道图像。测试表明，该设备能够独立采集纳摩尔浓度的荧光染料Cy5.5和Cy7。评估了近红外通道之间的串扰情况，部分串扰是常用有机荧光染料光谱中宽尾巴的结果，针对浓度/信号强度函数的线性范围进行了建模并进行了补偿。在两种荧光染料浓度相等的情况下，计算得出的补偿分别为近红外700和近红外780两个通道总信号强度的5.5%和22%。使用结肠腺瘤病小鼠模型，我们展示了在活体小鼠中可以同时、独立且重复地检测灌注和蛋白酶活性。所开发的设备应有助于对多种分子靶点进行体内成像。
