Department of Surgery, Division of Frontier Medical Sciences, Programs for Biomedical Research, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan.
Int J Colorectal Dis. 2014 Feb;29(2):217-23. doi: 10.1007/s00384-013-1791-9. Epub 2013 Nov 9.
A sporadic colon cancer mouse model with conditional mutations in adenomatous polyposis coli (Apc) is biologically relevant for human colorectal cancer (CRC). This study aimed to determine the utility and limitations of colonoscopy for evaluating colon tumors in this mouse model.
We compared the estimates of location, size, and miss rate of tumors detected during colonoscopy with those determined by necropsy. Sixty-six CPC-Apc mice originating from Apc (F/wt) mice harbor a Cdx2-Cre transgene in which colorectal tumorigenesis was driven by Apc allelic loss. The sensitivity and specificity of colonoscopy for detecting tumors in a mouse CRC model were investigated.
A strong positive correlation was found between tumor location as measured by colonoscopy and the location determined by necropsy (p < 0.001). A total of 120 tumors were graded during colonoscopy (grades 1-5: 0, 8, 20, 27, and 65 lesions, respectively), and a strong positive correlation was found between the tumor grade determined by colonoscopy and size measured by necropsy (grades 2-5: 2.08, 2.98, 4.02, and 5.09 mm, respectively; p < 0.005). Although the miss rate was 47.1 %, most of the missed tumors (96 %) were in close proximity (within 5 mm) of another tumor.
A colonoscopic method for the reliable measurement of colorectal tumors in vivo has been established. The application of this technique to mouse models of colon carcinogenesis will provide a better understanding of the dynamics of tumor growth.
具有腺瘤性结肠息肉病 coli(Apc)条件性突变的散发性结肠癌小鼠模型与人类结直肠癌 (CRC)具有生物学相关性。本研究旨在确定结肠镜检查在该小鼠模型中评估结肠肿瘤的适用性和局限性。
我们比较了结肠镜检查时检测到的肿瘤的位置、大小和漏诊率与尸检确定的肿瘤的位置、大小和漏诊率。66 只 CPC-Apc 小鼠源自 Apc(F/wt) 小鼠，携带 Cdx2-Cre 转基因，其中结直肠肿瘤发生是由 Apc 等位基因缺失驱动的。研究了结肠镜检查在小鼠 CRC 模型中检测肿瘤的敏感性和特异性。
结肠镜检查测量的肿瘤位置与尸检确定的位置之间存在很强的正相关关系（p<0.001）。共对 120 个肿瘤进行了结肠镜分级（1-5 级：0、8、20、27 和 65 个病变，分别），并且结肠镜检查确定的肿瘤分级与尸检测量的肿瘤大小之间存在很强的正相关关系（2-5 级：2.08、2.98、4.02 和 5.09 毫米，分别；p<0.005）。尽管漏诊率为 47.1%，但大多数漏诊的肿瘤（96%）都靠近另一个肿瘤（距离 5 毫米以内）。
建立了一种可靠测量体内结直肠肿瘤的结肠镜方法。该技术在结直肠癌发生的小鼠模型中的应用将更好地了解肿瘤生长的动力学。
