Ferreira J L, Eliasberg S J, Edmonds P, Harrison M A
US Food and Drug Administration, 60 Eighth Street N.E., Atlanta, Georgia 30309, USA.
J Food Prot. 2004 Jan;67(1):203-6. doi: 10.4315/0362-028x-67.1.203.
Samples of chili linked to a foodborne illness outbreak of type A botulism were examined for preformed type A botulinal toxin using two enzyme-linked immunosorbent assay (ELISA) procedures and the mouse bioassay. One of the samples was positive for type A botulinal toxin and three of the samples were negative for type A, B, E, and F botulinal toxins using the three methods. The mouse bioassay indicated that type A toxin was present at the 10,000 minimal lethal dose per gram (MLD per g) of product. The ELISA tests indicated a toxicity of 7,650 MLD per g with one method and 8,350 MLD per g with the other method. The sample toxicity determined by the ELISA was estimated by comparing samples to a standard curve generated with standard type A neurotoxin in casein buffer. The ELISA methods are more rapid than the mouse bioassay, since the toxin type can be determined in 1 day. The mouse bioassay is more sensitive than the ELISA but usually requires multiple assays to obtain the toxin type and toxicity. Type A culture isolates from the sample were also verified using one ELISA method.
对与A型肉毒中毒食源性疾病暴发相关的辣椒样本，采用两种酶联免疫吸附测定（ELISA）方法和小鼠生物测定法检测其中预先形成的A型肉毒毒素。其中一个样本的A型肉毒毒素呈阳性，使用这三种方法检测，另外三个样本的A型、B型、E型和F型肉毒毒素均为阴性。小鼠生物测定表明，每克产品中A型毒素的含量为10,000最小致死剂量（每克MLD）。ELISA检测一种方法显示每克毒性为7,650 MLD，另一种方法为8,350 MLD。ELISA测定的样本毒性是通过将样本与在酪蛋白缓冲液中用标准A型神经毒素生成的标准曲线进行比较来估计的。ELISA方法比小鼠生物测定更快，因为毒素类型可在1天内确定。小鼠生物测定比ELISA更灵敏，但通常需要多次测定才能确定毒素类型和毒性。还使用一种ELISA方法对样本中的A型培养分离株进行了验证。
