Miyasaka Natália Reiko Sato, Thuler Daniela Strauss, Floh Eny Iochevet Segal, Handro Walter, Toledo Mariana Braga Duarte, Gagioti Sônia Maria, Barbosa Heloiza Ramos
Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo. Av. Prof. Lineu Prestes, 1374, CEP-05508-900, São Paulo, Brasil.
Microbiol Res. 2003;158(4):309-15. doi: 10.1078/0944-5013-00209.
Beijerinckia derxii, a free-living nitrogen-fixing bacterium, maintained an increasing nitrogenase specific activity during the stationary growth phase. To verify the destination of the nitrogen fixed during this phase, intra and extracellular nitrogenated contents were analyzed. Organic nitrogen and amino acids were detected in the supernatant of the cultures. An increase in intracellular content of both nitrogen and protein occurred. Cytoplasmic granules indicated the presence of arginine. The ability of a non-diazotrophic bacterium (E. coli) to use B. derxii proteins as a source of nitrogen was observed concomitantly with E. coli growth. There is a suggestion that B. derxii contributes to the environment by both releasing nitrogenated substances and accumulating substances capable of being consumed after its death.
德氏拜叶林克氏菌（Beijerinckia derxii）是一种自生固氮细菌，在稳定生长期其固氮酶比活性不断增加。为了验证此阶段固定氮的去向，对细胞内和细胞外的含氮物质进行了分析。在培养物的上清液中检测到了有机氮和氨基酸。细胞内氮和蛋白质的含量均有所增加。细胞质颗粒表明存在精氨酸。在大肠杆菌生长的同时，观察到非固氮细菌（大肠杆菌）利用德氏拜叶林克氏菌的蛋白质作为氮源的能力。有人提出，德氏拜叶林克氏菌通过释放含氮物质和积累死后可供消耗的物质对环境做出贡献。
