Colina Rodney, Casane Didier, Vasquez Silvia, García-Aguirre Laura, Chunga Ausberto, Romero Héctor, Khan Baldip, Cristina Juan
Laboratorio de Virología Molecular, Centro de Investigaciones Nucleares, Facultad de Ciencias, Iguá 4225, 11400 Montevideo, Uruguay.
Populations, Génétique et Evolution, CNRS, 91198 Gif-sur-Yvette, France.
J Gen Virol. 2004 Jan;85(Pt 1):31-37. doi: 10.1099/vir.0.19472-0.
Hepatitis C virus (HCV) has high genomic variability and, since its discovery, at least six different types and an increasing number of subtypes have been reported. Genotype 1 is the most prevalent genotype found in South America. In the present study, three different genomic regions (5'UTR, core and NS5B) of four HCV strains isolated from Peruvian patients were sequenced in order to investigate the congruence of HCV genotyping for these three genomic regions. Phylogenetic analysis using 5'UTR-core sequences found strain PE22 to be related to subtype 1b. However, the same analysis using the NS5B region found it to be related to subtype 1a. To test the possibility of genetic recombination, phylogenetic studies were carried out, revealing that a crossover event had taken place in the NS5B protein. We discuss the consequences of this observation on HCV genotype classification, laboratory diagnosis and treatment of HCV infection.
丙型肝炎病毒（HCV）具有高度的基因组变异性，自发现以来，已报道了至少六种不同类型以及越来越多的亚型。1型是在南美洲发现的最常见基因型。在本研究中，对从秘鲁患者分离出的四株HCV毒株的三个不同基因组区域（5'UTR、核心区和NS5B）进行了测序，以研究这三个基因组区域HCV基因分型的一致性。使用5'UTR-核心序列进行的系统发育分析发现毒株PE22与1b亚型相关。然而，使用NS5B区域进行的相同分析发现它与1a亚型相关。为了测试基因重组的可能性，进行了系统发育研究，结果显示在NS5B蛋白中发生了一次交叉事件。我们讨论了这一观察结果对HCV基因型分类、实验室诊断和HCV感染治疗的影响。
