Kabakçı Alagöz Gökçe, Karataylı Senem Ceren, Karataylı Ersin, Celik Esra, Keskin Onur, Dinç Bedia, Cınar Kubilay, Idilman Ramazan, Yurdaydın Cihan, Bozdayı A Mithat
Institute of Hepatology, Ankara University Faculty of Medicine, Ankara, Turkey.
Turk J Gastroenterol. 2014 Aug;25(4):405-10. doi: 10.5152/tjg.2014.7083.
BACKGROUND/AIMS: Hepatitis C virus (HCV) genotyping has a considerable effect on therapy. The aim was to determine the change in prevalence of HCV genotypes in Turkey during the last decade and to compare the performance of DNA sequencing of different targets in the HCV genome (NS5B, E1, and 5'UTR).
Five hundred HCV RNA-positive patients (226 males, 274 females) were included in the study. The NS5B, E1, and 5'UTR regions of the HCV genome were amplified by polymerase chain reaction (PCR) in patients where possible. Amplified PCR products were sequenced directly, and phylogenetic analysis was performed. A commonly used database, namely www.hcv.lanl.gov, was also used to determine the genotypes.
Phylogenetic analysis of the NS5B, E1, and 5'UTR regions showed that 1b was the most frequent genotype, with percentages of 92.5%, 93.5%, and 87.7%, respectively. Genotype 1a was the second most prevalent genotype, with ratios of 6.7%, 5.6%, and 6.6%, whereas genotype 2a was detected in proportions of 0.4%, 0.2%, and 0.8%, respectively. Genotype 5 or 6 was not detected among patients. The phylogenetic analysis showed discordant results with 18 patients' genotypes for different targets. The phylogenetic analysis showed similar results with the hcv.lanl.gov database for the E1 and NS5B sequences.
There has been no change in genotyping profiles of Turkey during the last decade, representing 1b as the most prevalent subtype, followed by 1a. Phylogenetic analysis of HCV indicated high performance compared with the hcv.lanl.gov database when sequences of E1 and NS5B regions were analyzed.
背景/目的：丙型肝炎病毒（HCV）基因分型对治疗有相当大的影响。目的是确定过去十年土耳其HCV基因型流行率的变化，并比较HCV基因组不同靶点（NS5B、E1和5'UTR）的DNA测序性能。
本研究纳入了500例HCV RNA阳性患者（男性226例，女性274例）。尽可能对患者的HCV基因组NS5B、E1和5'UTR区域进行聚合酶链反应（PCR）扩增。对扩增的PCR产物直接进行测序，并进行系统发育分析。还使用了一个常用数据库，即www.hcv.lanl.gov来确定基因型。
对NS5B、E1和5'UTR区域的系统发育分析表明，1b是最常见的基因型，分别占92.5%、93.5%和87.7%。基因型1a是第二常见的基因型，比例分别为6.7%、5.6%和6.6%，而基因型2a的检测比例分别为0.4%、0.2%和0.8%。患者中未检测到基因型5或6。系统发育分析显示，18例患者不同靶点的基因型结果不一致。E1和NS5B序列的系统发育分析与hcv.lanl.gov数据库的结果相似。
过去十年土耳其的基因分型谱没有变化，1b是最常见的亚型，其次是1a。对E1和NS5B区域序列进行分析时，HCV的系统发育分析与hcv.lanl.gov数据库相比表现出高性能。
