Aruin L I, Balabolkin I I, Gershman G B, Subbotina O A, Shcherbakov P L, Sofonov A B
Arkh Patol. 1992;54(6):20-5.
Biopsies of the jejunum from 61 children, 6 months-4 years of age, including 35 with allergy to corn protein, 18 with celiac disease and 8 children without stomach and intestine pathology were studied. Biopsies were fixed in 10% formalin or in Carnois fixative, the sections were stained with hematoxylin and eosin, 0.5% toluidine blue (pH 0.5), and immunoperoxidase method was used for revealing IgA. The structure of the mucosa was not changed in the allergic patients, the content of the granulated and ungranulated labrocytes was increased, their number being decreased in the elimination diet. The number of plasma cells was diminished, and in only few of them IgA was found in children with severe alimentary malabsorption. A selective deficiency of IgA was diagnosed which determines the therapy of such children. Hyperregenerative atrophy of the mucous membrane and predominance of degranulated cells over undergranulated were noted in celiac disease.
对61名6个月至4岁儿童的空肠活检组织进行了研究，其中包括35名对玉米蛋白过敏的儿童、18名患有乳糜泻的儿童以及8名无胃肠疾病的儿童。活检组织用10%福尔马林或卡诺氏固定液固定，切片用苏木精和伊红、0.5%甲苯胺蓝（pH 0.5）染色，并用免疫过氧化物酶法检测IgA。过敏患者的黏膜结构未改变，颗粒状和非颗粒状淋巴细胞的含量增加，在排除饮食期间其数量减少。浆细胞数量减少，在严重营养吸收不良的儿童中，仅少数浆细胞中发现IgA。诊断出IgA选择性缺乏，这决定了此类儿童的治疗方法。乳糜泻患者可见黏膜的过度再生性萎缩以及脱颗粒细胞多于未脱颗粒细胞。
