用于实时检测和定量循环细胞的体内流式细胞仪。
In vivo flow cytometer for real-time detection and quantification of circulating cells.
作者信息
Novak J, Georgakoudi I, Wei X, Prossin A, Lin C P
机构信息
Wellman Laboratory of Photomedicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts 02114, USA.
出版信息
Opt Lett. 2004 Jan 1;29(1):77-9. doi: 10.1364/ol.29.000077.
Abstract
An in vivo flow cytometer is developed that allows the real-time detection and quantification of circulating fluorescently labeled cells in live animals. A signal from a cell population of interest is recorded as the cells pass through a slit of light focused across a blood vessel. Confocal detection of the excited fluorescence allows continuous monitoring of labeled cells in the upper layers of scattering tissue, such as the skin. The device is used to characterize the in vivo kinetics of red and white blood cells circulating in the vasculatúre of the mouse ear. Potential applications in biology and medicine are discussed.
摘要
一种体内流式细胞仪被开发出来，它能够实时检测和定量活体动物体内循环的荧光标记细胞。当感兴趣的细胞群体通过聚焦在血管上的狭缝光时，记录来自该细胞群体的信号。共聚焦检测激发荧光可对诸如皮肤等散射组织上层的标记细胞进行连续监测。该设备用于表征在小鼠耳部血管中循环的红细胞和白细胞的体内动力学。讨论了其在生物学和医学中的潜在应用。
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