Steen H B, Lindmo T
Cytometry. 1985 Jul;6(4):281-5. doi: 10.1002/cyto.990060402.
Light-scattering histograms of blood cell suspensions were recorded for various ranges of scattering angles by means of an arc-lamp-based flow cytometer (AL-FCM). The results were compared with those obtained with a conventional, laser-based flow cytometer (L-FCM) with forward scattering (2-20 degrees) and scattering at right angles. Measuring with the AL-FCM in the angle range upward from 13 degrees, the relative light-scattering intensities of lymphocytes, monocytes, and granulocytes were essentially independent of scattering angle and closely similar to the values measured as right-angle scattering in the L-FCM. With a range of scattering angles upward from 2 degrees the AL-FCM yielded histograms similar although not identical to that of the forward-scattering detector in the L-FCM. Differentiation between live and dead cells in this mode of operation was similar in the two instruments.
利用基于弧光灯的流式细胞仪（AL-FCM）记录血细胞悬液在不同散射角范围内的光散射直方图。将结果与使用传统的基于激光的流式细胞仪（L-FCM）在向前散射（2 - 20度）和直角散射情况下获得的结果进行比较。在13度及以上角度范围内使用AL-FCM进行测量时，淋巴细胞、单核细胞和粒细胞的相对光散射强度基本与散射角无关，并且与在L-FCM中作为直角散射测量的值非常相似。在2度及以上的散射角范围内，AL-FCM产生的直方图与L-FCM中向前散射探测器产生的直方图相似但不完全相同。在这种操作模式下，两种仪器对活细胞和死细胞的区分相似。
