肿瘤坏死因子-α和白细胞介素-8在兔颞下颌关节抗原诱导性关节炎中的作用。
Involvement of tumor necrosis factor-alpha and interleukin-8 in antigen-induced arthritis of the rabbit temporomandibular joint.
作者信息
Sukedai Miho, Tominaga Kazuhiro, Habu Manabu, Matsukawa Akihiro, Nishihara Tatsuji, Fukuda Jinichi
机构信息
First Department of Oral and Maxillofacial Surgery, Kyushu Dental College, 2-6-1 Manazuru, Kitakyushu 803-8580, Japan.
出版信息
J Oral Pathol Med. 2004 Feb;33(2):102-10. doi: 10.1111/j.1600-0714.2004.00008.x.
BACKGROUND
In temporomandibular joint (TMJ) arthritis, knowledge is limited about the source of the inflammatory mediators. The aim of this study was to investigate the immunohistochemical role of tumor necrosis factor-alpha (TNF-alpha) and interleukin-8 (IL-8) in the development of the antigen-induced arthritis of the rabbit TMJ.
METHODS
Unilateral TMJ arthritis was induced in 28 adult rabbits. From 6 h to 6 weeks after induction of arthritis, the topology of TNF-alpha and IL-8 was observed.
RESULTS
Positive reaction for TNF-alpha of synovial cells was observed within 3 days after induction and at 3 weeks after induction. TNF-alpha positive vascular endothelial cells and chondrocytes were identified throughout the observation period. IL-8 was detected only during the acute stage.
CONCLUSIONS
The cytokines TNF-alpha and IL-8 were observed in specific cells depending on the stage. TNF-alpha was particularly related with angiogenesis and cartilage destruction and IL-8 was involved in the acute stage of inflammation.
背景
在颞下颌关节（TMJ）关节炎中，关于炎症介质来源的知识有限。本研究的目的是调查肿瘤坏死因子-α（TNF-α）和白细胞介素-8（IL-8）在兔TMJ抗原诱导性关节炎发展过程中的免疫组织化学作用。
方法
在28只成年兔中诱导单侧TMJ关节炎。在关节炎诱导后6小时至6周，观察TNF-α和IL-8的拓扑结构。
结果
诱导后3天内及诱导后3周观察到滑膜细胞TNF-α阳性反应。在整个观察期内均鉴定出TNF-α阳性血管内皮细胞和软骨细胞。仅在急性期检测到IL-8。
结论
细胞因子TNF-α和IL-8在特定细胞中依阶段而出现。TNF-α尤其与血管生成和软骨破坏有关，而IL-8参与炎症急性期。
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