Versonnen Bram J, Goemans Geert, Belpaire Claude, Janssen Colin R
Ghent University, Laboratory of Environmental Toxicology and Aquatic Ecology, J Plateaustraat 22, B-9000 Gent, Belgium.
Environ Pollut. 2004;128(3):363-71. doi: 10.1016/j.envpol.2003.09.013.
As part of a large-scale monitoring program of bioaccumulating contaminants in the European eel (Anguilla anguilla) in Flanders (Belgium), we investigated potential effects of xenoestrogens in these fish. The present paper describes the results of the plasma vitellogenin (VTG) content, measured in 142 eels sampled at 20 different locations, in relation to the internal pollution levels. To validate the blood VTG assays, a small number of eels (n=8) was exposed to 10 microg ethinylestradiol/l (EE2) for 9 days. In this experiment, VTG was detected as a protein with a molecular weight of 214 kDa and confirmed by Western blotting. Compared with the solvent controls, significantly higher concentrations of VTG were measured in EE2 exposed eel. However, the VTG content was relatively low compared with other fish species exposed to high concentrations of estrogens. The plasma VTG content of eels from the field study was very low, despite a very high internal load of endocrine disrupters. These results, together with previously published studies, suggest that immature yellow European eel might not be the best sentinel species to study the effects of estrogenic compounds on VTG levels of wild fish populations.
作为比利时法兰德斯地区对欧洲鳗鲡（Anguilla anguilla）体内生物累积性污染物进行大规模监测项目的一部分，我们研究了这些鱼类体内外源性雌激素的潜在影响。本文描述了在20个不同地点采集的142条鳗鱼血浆中卵黄蛋白原（VTG）含量的测定结果及其与体内污染水平的关系。为验证血液VTG检测方法，将少量鳗鱼（n = 8）暴露于10微克/升乙炔雌二醇（EE2）中9天。在该实验中，检测到VTG是一种分子量为214 kDa的蛋白质，并通过蛋白质免疫印迹法得到确认。与溶剂对照组相比，暴露于EE2的鳗鱼体内VTG浓度显著更高。然而，与其他暴露于高浓度雌激素的鱼类相比，鳗鱼体内VTG含量相对较低。尽管野外研究中鳗鱼体内内分泌干扰物的负荷非常高，但其血浆VTG含量却非常低。这些结果与之前发表的研究共同表明，未成熟的欧洲黄鳗可能并非研究雌激素化合物对野生鱼类种群VTG水平影响的最佳指示物种。
