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通过使用同时表达CD4受体和CXCR4/CCR5共受体的HeLa细胞的检测方法鉴定出的1型人类免疫缺陷病毒的分支间中和作用及增强作用。

Interclade neutralization and enhancement of human immunodeficiency virus type 1 identified by an assay using HeLa cells expressing both CD4 receptor and CXCR4/CCR5 coreceptors.

作者信息

Barin Francis, Brunet Sylvie, Brand Denys, Moog Christiane, Peyre Robert, Damond Florence, Charneau Pierre, Barre-Sinoussi Françoise

机构信息

Laboratoire de Virologie, EA 3250, Université François Rabelais, Tours, France.

出版信息

J Infect Dis. 2004 Jan 15;189(2):322-7. doi: 10.1086/380099.

DOI:10.1086/380099
PMID:14722898
Abstract

We report on the development and evaluation of a human immunodeficiency virus (HIV) neutralization assay that uses P4P cells, which are CD4+CXCR4+CCR5+ HeLa cells that carry the lacZ gene under the control of the HIV-1 long terminal repeat. The results of the present study suggest that the P4P assay can be used for the extensive study of both neutralizing and enhancing activity in serum samples from HIV-1-infected patients and from vaccinated individuals.

摘要

我们报告了一种使用P4P细胞的人类免疫缺陷病毒（HIV）中和试验的开发和评估，P4P细胞是携带在HIV-1长末端重复序列控制下的lacZ基因的CD4+CXCR4+CCR5+ 人宫颈癌细胞。本研究结果表明，P4P试验可用于广泛研究来自HIV-1感染患者和接种疫苗个体的血清样本中的中和及增强活性。

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