Geluk A, van Meijgaarden K E, Franken K L M C, Wieles B, Arend S M, Faber W R, Naafs B, Ottenhoff T H M
Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands.
Scand J Immunol. 2004 Jan;59(1):66-70. doi: 10.1111/j.0300-9475.2004.01358.x.
Mycobacterium tuberculosis culture filtrate protein-10 (CFP-10) (Rv3874) is considered a promising antigen for the immunodiagnosis of tuberculosis (TB) together with early secreted antigens of M. tuberculosis (ESAT-6). Both ESAT-6 and CFP-10 are encoded by the RD1 region that is deleted from all tested M. bovis bacille Calmette-Guérin (BCG) strains but present in M. leprae, M. tuberculosis, M. bovis, M. kansasii, M. africanum and M. marinum. In this study, the homologue of CFP-10 in M. leprae (ML0050) is identified and characterized. Interferon-gamma production in response to this homologue by T cells from leprosy patients, TB patients and unexposed controls shows that CFP-10 of M. leprae is a potent antigen that crossreacts with CFP-10 of M. tuberculosis at the T-cell level. This crossreactivity has implications for the use of CFP-10 of these mycobacterial species as diagnostic tool in areas endemic for both the diseases.
结核分枝杆菌培养滤液蛋白10（CFP-10）（Rv3874）与结核分枝杆菌早期分泌抗原（ESAT-6）一起被认为是用于结核病免疫诊断的一种有前景的抗原。ESAT-6和CFP-10均由RD1区域编码，该区域在所有测试的卡介苗（BCG）菌株中缺失，但存在于麻风分枝杆菌、结核分枝杆菌、牛分枝杆菌、堪萨斯分枝杆菌、非洲分枝杆菌和海分枝杆菌中。在本研究中，鉴定并表征了麻风分枝杆菌中CFP-10的同源物（ML0050）。麻风病患者、结核病患者和未接触过的对照的T细胞对该同源物产生的γ干扰素表明，麻风分枝杆菌的CFP-10是一种在T细胞水平上与结核分枝杆菌的CFP-10发生交叉反应的强效抗原。这种交叉反应性对于在这两种疾病的流行地区将这些分枝杆菌物种的CFP-10用作诊断工具具有重要意义。
