Moore S, Ide M, Randhawa M, Walker J J, Reid J G, Simpson N A B
Department of Periodontology and Preventive Dentistry, Guy's, King's, and St Thomas' Dental Institute, King's College, London, UK.
BJOG. 2004 Feb;111(2):125-32. doi: 10.1046/j.1471-0528.2003.00024.x-i1.
To investigate a putative relationship between preterm delivery and the carriage of polymorphic genes that code for the cytokines interleukin-1beta (IL-1beta) at codon +3953 and tumour necrosis factor-alpha (TNF-alpha) at codon -308 in a group of postpartum women and to elucidate if the concurrent presence of periodontal disease increased the risk of preterm delivery in this group.
Case-control study
Postnatal wards at Guy's and St Thomas' Hospital Trust.
Postpartum women from southeast London, UK.
Case subjects were defined as those who experienced a birth at less than 37 weeks of gestation. Control subjects gave birth at term. Demographic data were collected and a periodontal examination was performed. Blood samples were collected and analysed by restriction fragment length polymerase techniques for the presence of each of the allelic variants.
The level of periodontal disease and the carriage of allelic variants of IL-1beta+3953 and TNF-alpha-308 genes.
Forty-eight case subjects and 82 control subjects were assessed. There was no statistically significant difference in the carriage of the IL-1beta+3953 allelic variant between cases and controls (29%versus 18%, P= 0.112). However, 23 (48%) of the case subjects and 24 (29%) of controls were heterozygous or homozygous for the variant TNF-alpha-308 gene (odds ratio [OR] 2.2, 95% confidence interval [CI] 1.0-5.0, P= 0.026). There was no association between the carriage of either the polymorphic IL-1beta+3953 or TNF-alpha-308 variant and the severity of periodontal disease. The combination of periodontal disease and the allelic variant did not increase the risk of preterm delivery.
In this study, a higher proportion of women who delivered preterm carried the polymorphic TNF-alpha-308 gene. There did not appear to be any interaction between either of the genotypes and periodontal disease with preterm delivery as has been reported for bacterial vaginosis and the TNF-alpha-308 polymorphic gene.
调查一组产后妇女中早产与编码细胞因子白细胞介素-1β（IL-1β）第+3953位密码子和肿瘤坏死因子-α（TNF-α）第-308位密码子的多态性基因携带情况之间的假定关系，并阐明牙周病的同时存在是否会增加该组妇女早产的风险。
病例对照研究
盖伊和圣托马斯医院信托基金的产后病房。
来自英国伦敦东南部的产后妇女。
病例组定义为妊娠少于37周分娩的妇女。对照组为足月分娩的妇女。收集人口统计学数据并进行牙周检查。采集血样，采用限制性片段长度聚合酶技术分析各等位基因变体的存在情况。
牙周病水平以及IL-1β+3953和TNF-α-308基因等位基因变体的携带情况。
评估了48例病例组和82例对照组。病例组和对照组之间IL-1β+3953等位基因变体的携带情况无统计学显著差异（29%对18%，P=0.112）。然而，23例（48%）病例组和24例（29%）对照组为TNF-α-308基因变体的杂合子或纯合子（比值比[OR]2.2，95%置信区间[CI]1.0-5.0，P=0.026）。多态性IL-1β+3953或TNF-α-308变体的携带与牙周病严重程度之间无关联。牙周病与等位基因变体的组合并未增加早产风险。
在本研究中，早产妇女中携带多态性TNF-α-308基因的比例更高。与细菌性阴道病和TNF-α-308多态性基因的报道不同，这两种基因型与牙周病和早产之间似乎没有任何相互作用。
