Marini Marina, Lapalombella Rosa, Canaider Silvia, Farina Antonio, Monti Daniela, De Vescovi Valentina, Morellini Marina, Bellizzi Dina, Dato Serena, De Benedictis Giovanna, Passarino Giuseppe, Moresi Raffaella, Tesei Silvia, Franceschi Claudio
Institute of Histology and General Embryology, University of Bologna, Via Belmerloro 8, Bologna 40126, Italy.
Exp Gerontol. 2004 Jan;39(1):83-90. doi: 10.1016/j.exger.2003.09.023.
'Successful aging', i.e. the ability to attain old age in relatively good health, is believed to be related to the capability to cope with different environmental stresses. Independently of their specific differentiation, all body cells respond to hyperthermia and other stresses with the production of Heat Shock Proteins (HSPs) that play an important role in cell survival. We investigated the heat shock response in B-lymphoid cell lines from 44 centenarians and 23 younger subjects, by studying both HSP70 synthesis and cell survival after hyperthermic treatment. Interestingly, no significant difference could be found between the two age groups as far as HSP70 synthesis was concerned; moreover, cell lines from centenarians appeared to be less prone to heat-induced apoptosis than lines from younger controls. These results, which are in contrast with previous findings showing an age-related decrease of the HSP70 synthesis and of hyperthermic response, corroborate the above mentioned hypothesis that the biological success of centenarians is due to the preservation of the capability to cope with stresses. An A/C polymorphism identified in the promoter region of HSP70-1 gene had been previously shown to affect the probability to attain longevity in females. To investigate if this effect was related to any influence of this polymorphism on HSP70 protein synthesis the correlation between A/C polymorphism and protein synthesis was investigated. We found that cells from AA centenarian females displayed a lower synthesis of HSP70.
“成功老龄化”，即相对健康地步入老年的能力，被认为与应对不同环境压力的能力有关。无论其具体分化如何，所有体细胞都会通过产生热休克蛋白（HSPs）来应对高温和其他压力，而热休克蛋白在细胞存活中起着重要作用。我们通过研究热疗后HSP70的合成以及细胞存活情况，调查了44名百岁老人和23名较年轻受试者的B淋巴细胞系中的热休克反应。有趣的是，就HSP70的合成而言，两个年龄组之间未发现显著差异；此外，与年轻对照组的细胞系相比，百岁老人的细胞系似乎更不易发生热诱导凋亡。这些结果与之前显示HSP70合成及热休克反应随年龄下降的研究结果相反，证实了上述假设，即百岁老人的生物学成功归因于应对压力能力的保留。先前已表明，在HSP70 - 1基因启动子区域鉴定出的A/C多态性会影响女性达到长寿的概率。为了研究这种效应是否与该多态性对HSP70蛋白合成的任何影响有关，我们调查了A/C多态性与蛋白合成之间的相关性。我们发现，AA基因型百岁女性的细胞显示出较低的HSP70合成。
