Zhao Ke-Seng, Jin Chunhua, Huang Xuliang, Liu Jie, Yan Wen Sheng, Huang Qiaobing, Kan Wenhong
Department of Pathophysiology, First Military Medical University, Guangzhou 510515, China.
Clin Hemorheol Microcirc. 2003;29(3-4):211-7.
Polydatin is extracted from a traditional Chinese herbal medicine, Polygonum cuspidatum, and has a special effect in shock treatment. The aim of this study is to explain the cellular and molecular basis of Polydatin in shock treatment. The fluorescent probe techniques, patch clamp method, and cellular flow chamber were used to test intracellular variables of vascular smooth muscle cells (VSMC), myocardial cells (MC), endothelial cells (EC), and white blood cell (WBC). It was shown that Polydatin could inhibit ICAM-1 expression in EC stimulated by lipopolysaccharide (LPS), attenuate WBC-EC adhesion, increase [Ca2+]i in MC with enhancement of MC contraction extent, activate KATP channels of VSMC, and decrease pHi value and [Ca2+]i of VSMC in shock. The study suggests that Polydatin has multiple effects on VSMC, MC, WBC and EC, which are related to the enhancement of heart function and improvement of microcirculatory perfusion in shock.
虎杖苷是从传统中药虎杖中提取的，在休克治疗中具有特殊作用。本研究的目的是解释虎杖苷在休克治疗中的细胞和分子基础。采用荧光探针技术、膜片钳方法和细胞流动腔检测血管平滑肌细胞（VSMC）、心肌细胞（MC）、内皮细胞（EC）和白细胞（WBC）的细胞内变量。结果表明，虎杖苷可抑制脂多糖（LPS）刺激的EC中细胞间黏附分子-1（ICAM-1）的表达，减弱WBC与EC的黏附，增加MC中的[Ca2+]i并增强MC的收缩程度，激活VSMC的ATP敏感性钾通道（KATP通道），并降低休克时VSMC的细胞内pH值（pHi）和[Ca2+]i。该研究表明，虎杖苷对VSMC、MC、WBC和EC具有多种作用，这与休克时心功能增强和微循环灌注改善有关。
