Víg Julianna, Takács József, Vastagh Csaba, Baldauf Zsolt, Veisenberger Eleonóra, Hámori József
Neurobiology Research Group, United Research Organization of the Hungarian Academy of Sciences and Semmelweis University, Budapest, Hungary.
J Neurocytol. 2003 Mar;32(3):217-27. doi: 10.1023/B:NEUR.0000010081.54613.94.
The Lugaro cell is a feedback interneuron of the cerebellar cortex, recognizable by its characteristic morphology. Postnatal neuronal migration to the cortex has been described for several cerebellar interneurons. Since in our previous studies we observed Lugaro-like cells (LCs) in the white matter (WM) and internal granular layer (IGL) of the cerebellum of young cats, we assumed that a proportion of these cells migrate also postnatally to their destination. In the present study using and immunostaining for the metabotropic glutamate receptor mGluR1alpha and neurofilament protein SMI 311 the number and spatial distribution of LCs at different postnatal days were investigated. We found that the number and distribution of both mGluR1a-immunoreactive (ir) and of SMI 311-ir LCs changed with age in the developing cerebellar cortex of kittens: developing LCs express mGluR1alpha already in the newborn, while expression of SMI 311-ir in LCs appears only about a week later. At postnatal day 1 (P1) relatively few mGluR1-ir LCs were detected in the WM and at the border of WM and IGL. Later, their number increased sharply until P15 (6-7 fold) and decreased continuously between P15 and P135. SMI 311-ir LCs were not present at P1 and even at P8 only a few were observed in the WM or in infraganglionic positions. Their number increased gradually (12-14 fold) until adulthood when their number was stabilized at 8.000-10.000/cerebellum. At the same time the number of probably ectopic SMI 311-ir LCs decreased with age: at P22 about one third of them was found in "ectopic" position, whereas in the adult cat only about 10-12% of LCs's was either in the WM or scattered in the whole depth of the granular layer. These results suggest that: (1) most LCs appear in the cerebellar cortex postnatally; and (2) postnatal migration and incorporation of LCs to the cortex is a much longer process than previously expected, occurring even after the cytoarchitectonic built-up (about P65-P70 in cat) of the cerebellum.
卢加罗细胞是小脑皮质的一种反馈中间神经元，可通过其特征性形态识别。已有多种小脑中间神经元的出生后神经元向皮质迁移的描述。由于在我们之前的研究中，在幼猫小脑的白质（WM）和内颗粒层（IGL）中观察到了卢加罗样细胞（LCs），我们推测这些细胞中有一部分也是在出生后迁移到其目的地的。在本研究中，使用代谢型谷氨酸受体mGluR1α和神经丝蛋白SMI 311进行免疫染色，研究了不同出生后天数的LCs的数量和空间分布。我们发现，在小猫发育中的小脑皮质中，mGluR1a免疫反应性（ir）和SMI 311-ir LCs的数量和分布均随年龄变化：发育中的LCs在新生时就已表达mGluR1α，而LCs中SMI 311-ir的表达大约在一周后才出现。在出生后第1天（P1），在WM以及WM和IGL的边界处检测到相对较少的mGluR1-ir LCs。之后，它们的数量急剧增加，直到P15（增加6 - 7倍），并在P15和P135之间持续减少。P1时不存在SMI 311-ir LCs，甚至在P8时，在WM或神经节下位置仅观察到少数。它们的数量逐渐增加（12 - 14倍），直到成年时数量稳定在8000 - 10000个/小脑。同时，可能异位的SMI 311-ir LCs的数量随年龄减少：在P22时，约三分之一的细胞处于“异位 ”位置，而在成年猫中，只有约10 - 12%的LCs位于WM或分散在颗粒层的整个深度。这些结果表明：（1）大多数LCs在出生后出现在小脑皮质中；（2）LCs出生后向皮质的迁移和整合过程比先前预期的要长得多，甚至在小脑的细胞结构形成后（猫约在P65 - P70）仍会发生。
